苦参碱体外对兔晶状体上皮细胞细胞周期的影响  

Effect of matrine on cell cycle of rabbit lens epithelial cells in vitro

作　　者：王智[1] 何湘珍[1] 彭辉灿[1] 肖启国[1] 刘嘉毅[1] 

机构地区：[1]南华大学附属第二医院眼科,中国湖南省衡阳市421001

出　　处：《国际眼科杂志》2010年第2期233-234,共2页International Eye Science

摘　　要：目的:研究苦参碱(matrine,Mat)体外对兔晶状体上皮细胞细胞周期的影响。方法:将0.5,1.0和1.5g/L的苦参碱作用于体外培养的兔晶状体上皮细胞,通过流式细胞仪检测其细胞周期各时相的百分比。结果:不同浓度梯度的苦参碱作用体外培养的兔晶状体上皮细胞72h后,与对照组及各浓度的Mat组之间相互比较,随着苦参碱浓度增高,G0-G1期细胞数量明显增加(P<0.05),S期细胞明显减少(P<0.05),呈明显的剂量-效应关系。结论:苦参碱能明显抑制体外培养的兔晶状体上皮细胞的增殖活性和细胞有丝分裂。AIM .To study the effects of matrine （Mat） on cell cycle of rabbit lens epithelial cells （RLECs） in vitro. METHODS-0.5g, 1.0g and 1.5g/L Mat was added to the RLECs in vitro for 72 hours culture respectively. The cell cycle of RLECs was analyzed by flow cytometer （FCM）. RESULTS. The percentage of RLECs in S stage was decreased significantly by Mat （ P 〈 0.05 ）, while with a significant increase in the percentage of G0-G1 stage cells （P〈0.05）. CONCLUSION Mat could effectively prevent cell division to inhibit the proliferation of RLECs in vitro.

关 键 词：苦参碱 晶状体上皮细胞 细胞增生 后囊膜混浊 兔 细胞周期 

分 类 号：R285.5[医药卫生—中药学]