间歇低氧程度与频率对人脐静脉内皮细胞白介素6及白介素8影响的体外实验  

Effect of different intermittent hypoxia patterns on interleukin-6 and interleukin-8 levels of human umbilical vein endothelial cells

作　　者：李业梅[1] 陈宝元[1] 冯靖[1] 刘振宽[1] 董丽霞[1] 

机构地区：[1]天津医科大学总医院呼吸科,300052

出　　处：《中华耳鼻咽喉头颈外科杂志》2010年第2期139-142,共4页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：国家自然科学基金（30770934）

摘　　要：目的探讨不同程度和频率间歇低氧（intermittent hypoxia，IH）对血管内皮细胞炎性反应的影响及规律。方法将人脐静脉内皮细胞可传代细胞株ECV304植于培养舱，以模拟睡眠呼吸暂停模式低氧／复氧模式，将舱内气体由计算机程控为不同程度与频率IH环境。细胞株分为1个持续正常氧的对照组和8个实验组。不同IH程度分3组，频率固定为12次／h，即IH程度组1～3的低氧时O2体积分数分别为10％、1．5％及0．5％。不同IH频率分5组，固定低氧时O：体积分数为0．5％，频率组1～5的IH频率分别为40次／h、20次／h、12次／h、9次／h和6次／h。细胞暴露后以酶联免疫吸附法（enzyme linkedimmuno sorbent assay，ELISA）测定培养液中白细胞介素6（IL-6）及白细胞介素8（IL-8）质量浓度。结果IL-6和IL-8水平在3个不同程度IH组均高于正常O2组（F值分别为1961．100和103．855，P值均〈0．001），随IH程度加重IL-6及IL-8水平呈逐步升高趋势，不同程度IH组间两两比较，差异均有统计学意义（P值均〈0．05）。重度IH暴露，不同频率IH各组ECV304细胞的IL-6和IL-8水平不同（F值分别为544．396和149．328，P值均〈0．001）。随着IH频率降低IL-6及IL-8水平在20次／h组轻度升高（P〈0．05），至127X／h达最高（P〈0．05），9次／h略减低（P〈0．05），6b4：／h进一步减低（P〈0．05）。结论IH可造成血管内皮细胞的炎性损伤，且IL-6及IL-8分泌水平具低氧程度依赖性。重度低氧暴露下，IL-6和IL-8分泌水平有随频率下降而增加，而后减低的趋势。Objective To investigate the effect of different patterns of intermittent hypoxia on the levels of interleukin-6 （IL-6） and interleukin-8 （IL-8） of vascular endothelial cell. Methods The human umbilical vein endothelial cells of the line ECV304 were used to set up the cells model. The experiment cell strains contained one control group and eight experimental groups, 1 constance normoxia group （control group）, 3 different intermittent hypoxia （IH） degree groups （ 10% 02 group, 1.5% 02 group, 0. 5% 02 group with 12 times/h）, 4 different IH frequency groups （40 times/h, 20 times/h, 12 times/h, 9 times/h and 6 times/h with 0. 5% 02 ）. The specimen were put into a program-controlled gas delivery system with different level and frequency of IH respectively. Then enzyme linked immunosorbent assay （ELISA） was used to examine the levels of IL-6 and IL-8. Results Higher level of IL-6 and IL-8 were found in 3 different IH degree groups than in control group （F were 1961. 100 and 103. 855 respectively, P all 〈 0. 001 ）. The level of IL-6 and IL-8 was gradually increased with the decreasing of IH degree. The difference of IL-6 and IL-8 levels between each two different groups in IH degree was significant （ P all 〈 0. 05 ）. The level of IL-6 and IL-8 groups was significantly different in severe IH exposure （ F were 544. 396 and 149. 328 respectively, P all 〈0. 001 in both groups）. There was a trend as the IH frequency decreased, the level of IL-6 and IL-8 was tenderly increased in 20/hour group （ P 〈 0. 05 ）, highest in 12/hour group （ P 〈 0.05 ）, decreased in 9/hour group （ P 〈 0. 05 ）, and decreased further in 6/hour group （ P 〈 0. 05 ） in severe IH expose. There was statistical significance between each two different frequency IH groups （ P 〈 0. 05 ）. Conclusions Intermittent hypoxia caused significant inflammatory reaction in vascular endothelial ceils. The secreted level of IL-6 and IL-8 depended on the degree of hypoxia. There was a trend as the

关 键 词：缺氧 脐静脉 细胞系 白细胞介素6 白细胞介素8 

分 类 号：R766[医药卫生—耳鼻咽喉科]