大鼠肾下腹主动脉缺血再灌注对心肌肌浆网钙泵活性及其基因表达的影响  

Effects of activity and expression of sarcoplasmic reticulum Ca^(2+)-ATPase in rat heart in infrarenal abdominal aorta occlusion induced ischemia and reperfusion injury

作　　者：于影[1,2] 胡杰[2] 高琴[2] 汪思应[1] 

机构地区：[1]安徽医科大学病理生理学教研室,安徽合肥230032 [2]蚌埠医学院生理学教研室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2010年第2期112-114,共3页Journal of Bengbu Medical College

基　　金：安徽省高等学校省级自然科学研究资助项目(KJ2007B018)

摘　　要：目的:观察大鼠肾下腹主动脉缺血再灌注对心肌肌浆网钙泵(sarcoplasmic reticulum Ca2+-ATPase,SERCA)的活性及其基因表达的影响。方法:复制大鼠肾下腹主动脉缺血再灌注(ischemia and reperfusion,I/R)模型。将SD雄性大鼠随机分为假手术组,缺血30min分别再灌注2、12、24和48h(I/R2h组、I/R12h组、I/R24h组、I/R48h组)。采用无机磷比色法检测缺血再灌注不同时间点心肌肌浆网SERCA活性,反转录酶PCR技术测定SERCA基因的表达。结果:I/R24h组、I/R48h组SERCA的活性及基因表达均显著低于假手术组(P<0.01);I/R12h组SERCA的活性均低于假手术组(P<0.01),但基因表达无明显变化(P>0.05);I/R2h组SERCA的活性及其基因表达与假手术组差异均无统计学意义(P>0.05)。结论:肾下腹主动脉缺血再灌注过程严重影响SERCA的活性及其基因的表达。Objective：To investigate the sarcoplasmic reticulum Ca^2＋ -ATPase （SERCA） activity and mRNA expression in rat heart in infrarenal abdominal aorta occlusion induced ischemia and reperfusion injury （ I/R ）. Methods ： Forty male Sprague-Dawley rats were randomized into five groups：pseudo-operation group, I/R2h group, I/R12h group, I/R24h group and I/R48h group. Rats in pseudooperation group were undergone laparotomy only. Meanwhile, rats in I/R groups were reperfused with different hours （2,12,24 and 48 hours） following by 30 minutes infrarenal abdominal aorta occlusion induced ischemia. Then the rat hearts in each group were obtained at the specified time points. The activity of SERCA was deternfined by inorganic phosphorus colorimetry, and gene expression of SERCA was analyzed by reverse transcriptional-PCR. Results ： Compared with the pseudo-operation group, the levels of SERCA activity and gene expression in I/R24h group and I/R48h group were down-regulated significantly（ P 〈 0.01 ） ;but in I/R12h group, SERCA activity was decreased, while gene expression had no statistical difference. And there were no statistical significance about SERCA activity and gene expression in rat heart between the I/R2h group and pseudo-operation group （P 〉 0.05 ）. Conclusions： Infrarenal abdominal aorta occlusion may obviously affect the activity and gene expression of SERCA in rat heart.

关 键 词：心脏/生理学 再灌注损伤 大鼠 心肌 肌浆网钙泵 肾下腹主动脉 

分 类 号：R331.31[医药卫生—人体生理学]