水稻抗稻瘟病基因Pi-d2基因标签的建立与应用  

Establishment and Application of Gene Tagging Linked to Rice Blast Resistance Gene Pi-d2

作　　者：高利军[1,2] 邓国富[2] 高汉亮[2] 高国庆[2] 周萌[2] 周维永[2] 颜群[2] 张晋[2] 戴高兴[2] 

机构地区：[1]中国农业大学,北京100193 [2]广西农业科学院,广西南宁530007

出　　处：《西南农业学报》2010年第1期77-82,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家863计划子课题(2006AA100101-2);广西科技创新能力与条件建设项目(桂科能0815011-6-1-9);广西科技基础条件平台建设项目(09-007-08)

摘　　要：建立抗稻瘟病基因的分子标记对培育抗稻瘟病品种有重要意义。研究利用71个广西地方菌株检测地谷对稻瘟病菌的抗性,并利用地谷中Pi-d2基因与广恢998等位基因在基因组序列上一个碱基的差异,设计出以抗病基因本身序列为引物的基因标签M-Pid2,通过分析地谷与广恢998的F2群体的基因型与对稻瘟病抗感分离的吻合度来验证该标签的准确性,用M-Pid2扩增62份水稻种质验证该标签的特异性。结果表明,地谷能抗71个地方稻瘟病菌株中的59个菌株,抗性频率达83.1%。用M-Pid2扩增地谷与广恢998的F2群体的基因型与其对稻瘟病的抗感分离完全吻合;用M-Pid2扩增62份水稻种质,仅在地谷中扩增出629bp特异条带。从而确认抗稻瘟病基因Pi-d2为地谷的主效抗稻瘟病基因,且在广西有育种利用价值;标记M-Pid2能准确用于抗稻瘟病基因Pi-d2的辅助选择。The establishment of molecular marker of rice blast resistance genes was a powerful strategy in resistance to rice blast breeding. In the present study, seventy-one strains in Digu variety was used to check the regional rice blast resistance in Guangxi. A gene tagging M-Pid2 was amplified by the primers designed with the sequence of resistance gene itself, using a base difference between Pi-d2 gene in Digu variety and Guanghui 998 allele. The validity of the gene tag was tested in a F2 generation derived from Dign（a resistant variety including Pi-d2） and Guanghui 998 （ a susceptible restore line） and a group of germplasm containing 62 rice germplasm. The resistance spectrum was detected by inoculating Pi-d2 gene sponsor with 71 rice blast pathogen strains collected from Guangxi. The resistance spectrum of all materials were also detected in natural disease nursery located in Cenxi county. The results showed that the resistance indicated that Pi-d2 gene tag was highly consistent with that indicated under field detection. Furthermore, PCR analysis indicated that, among the 62 rice germplasm, a specific band of 629 bp was amplified only in Digu variety. It was concluded that rice blast resistance gene Pi-d2 was the main-effect blast resistance genes of Digu rice. and the gene tag could be credibly used in marker assisted selection and germplasm differentiation.

关 键 词：水稻 地谷 抗稻瘟病基园用Ri-d2 基因标签 

分 类 号：S511.034[农业科学—作物学] S855.9