青蒿植物的遗传转化研究  

Study on Genetic Transformation of Artemisia annua L.

作　　者：王跃华[1] 代勇 孙雁霞[1] 徐世军 王晓蓉 

机构地区：[1]成都大学生物产业学院,四川成都610016 [2]成都恩威集团(投资)有限公司,四川成都610041

出　　处：《西南农业学报》2010年第1期188-191,共4页Southwest China Journal of Agricultural Sciences

基　　金：国家科技支撑计划项目(2007BA127B03)

摘　　要：为了建立利用发根农杆菌诱导青蒿植物Artemisia annuaL.毛状根的培养系统,分别用发根农杆菌Ri1601、ATCC15834感染青蒿无菌苗的子叶、下胚轴、叶片、叶柄等外植体获得毛状根,并比较不同因素对毛状根诱导和生长的影响。发根农杆菌诱导青蒿毛状根的有效方法被建立,借此为青蒿大规模遗传转化提供了理论基础。To establish an effective system for genetic transformation ofArtemisia annua L. by Agrobacteriam rhizogenes, the hairy roots of Artemisia annua L was induced from infected explants such as cotyledon, hypocotyl, leaf and petiole with Agrobacterium rhizogenes Ril601 and ATCC15834. The effect of different factors on the induction and growth of hairy roots were compared. The results showed that the method for the induction of hairy roots ofArtemisia annua L. using Agrobaterium rhizogenes was established ,which could provide rationale for wide scale genetic transformation of Artemisia annua L.

关 键 词：青蒿 毛状根 发根农杆菌 

分 类 号：S567[农业科学—中草药栽培]