瘦素受体在小尾寒羊消化系统的表达  

Expression of Leptin Receptor in Digestive System of Small Tail Han Sheep

作　　者：肖欢[1] 崔亚利[1] 胡满[1] 邓艳幔[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001

出　　处：《中国农学通报》2010年第3期19-22,共4页Chinese Agricultural Science Bulletin

基　　金：河北农业大学博士基金资助项目(BS200618)

摘　　要：试验通过HE、免疫组织化学SABC染色和Western Blot方法对瘦素受体(ob-R)在小尾寒羊消化系统的表达进行研究。HE染色结果显示,各组织结构正常,细胞形态清晰可见;SABC染色结果显示,在皱胃胃体部固有层胃底腺的主细胞和壁细胞及十二指肠黏膜上皮细胞和固有层肠腺的柱状细胞中均可见大小数量不等的棕黄色颗粒;Western Blot检测发现,在皱胃和十二指肠均检测到120、110和98 ku三条带。120 ku长型瘦素受体蛋白在胃中表达量显著高于小肠中的表达;110 ku的短型瘦素受体蛋白在小肠和皱胃中表达量接近;98 ku短型受体蛋白在胃和小肠表达均较弱。试验结果表明,瘦素受体在小尾寒羊消化系统中表达,对调节能量平衡和饲料摄入具有重要作用。In the present study,we analysed expression of the leptin receptor in normal digestive system of Small Tail Han Sheep using Hematoxylin-eosin staining,immunohistochemistry and Western Blot.Our results demonstrate：The structures of the organs are normal,and the shapes of cells are clearly visible.There are lots of positive brown granules in Chief cells and Parietal cells in abomasum as well as the mucosa epithelial cells and gland cells of duodenum.Three bands with a molecular mass close to 120 ku,110 ku and 98 ku were identified by Western Blot.The ob-R levels of 120 ku in abomasum were significantly higher than that of in small intestine.The levels of 110 ku were similar in the two organs.The expression of 98 ku ob-R was weak. We inferred that Leptin receptor in digestive system can regulate the energy balance and appetite of Small Tail Han sheep.

关 键 词：OB-R 皱胃 十二指肠 免疫组织化学 WESTERN BLOT 

分 类 号：S826[农业科学—畜牧学]