基于绵羊皮肤组织ESTs开发新型微卫星标记  

Identification of Microsatellites from Skin of Sheep

作　　者：王遵宝[1] 努尔力.阿不力孜 赵宗胜[1] 曾献存[1] 吴洪宾[1] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832003

出　　处：《中国农学通报》2010年第3期42-45,共4页Chinese Agricultural Science Bulletin

基　　金：国际科技合作项目"绵羊非繁殖季节产羔性状的QTL定位及相关基因的分离"(编号DF2007DFB30420)资助

摘　　要：利用生物信息学方法,在从国际公共生物数据库检索获得绵羊皮肤组织相关的3295条EST序列中筛选微卫星标记。结果表明,从绵羊皮肤组织相关的EST序列中搜索到微卫星209个,含有微卫星的序列196个,占整个EST序列数据库的6.3%,其中双碱基重复150个,三碱基重复19个,四碱基重复12个,五碱基重复17个,六碱基重复11个。在这些微卫星序列中,AC/TG重复在双碱基类型中最丰富,占双碱基微卫星序列总数的67%。根据筛选到的微卫星序列设计并合成引物22对,其中18对引物有扩增产物,且条带清晰,6对引物在中国美利奴羊群内多态性丰富,平均PIC达0.7001,为后期与毛品质进行相关性分析,寻找毛用性状新型分子标记奠定了基础。3,295 ESTs from NCBI were mined to develop EST-SSR markers.By using SSRHunter soft tools,a total of 209 SSRs were identified from 196 ESTs.The frequency of EST-SSRs was 6.3%.The dinucleotide repeat motif was the most abundant SSR,accounting for 67%,followed by 9.1%,8.1%,5.7%and 5.3%, respectively,for tri-,hexa-,penta- and tetra-nucleotide repeats.Among the di-nucleotide repeats,AC/TG （31%）were the most abundant type.22 primer pairs were designed from microsatellite,18 pairs could show clear PCR products by electrophoresis,and 6 pairs show highly polymorphism in Chinese Merino sheep.The mean PIC value was 0.7001,ranging from 0.5499 to 0.7607.These EST-SSR markers can be used for further study such as in genetic mapping,identification of quantitative trait loci（QTLs）,and comparative genomics studies of sheep.

关 键 词：绵羊 皮肤 EST SSR 

分 类 号：S813.3[农业科学—畜牧学]