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作 者:韩超[1] 张金恒[1] 张守才[2] 周贵忠[1] 李大鹏[1]
机构地区:[1]青岛科技大学生态环境与农业信息化研究所环境与安全工程学院,山东青岛266042 [2]青岛市农业科学研究院,山东青岛266100
出 处:《中国农学通报》2010年第3期111-116,共6页Chinese Agricultural Science Bulletin
基 金:"863"课题(2007AA10Z205);国家自然科学基金(40601062)
摘 要:利用^(15)N示踪技术,研究水稻叶片全氮含量、^(15)N丰度的变化以及与施氮水平之间的关系,为基于叶片诊断水稻氮素营养提供研究基础。以3个水稻品种,临稻11,圣稻13和阳光200为材料,进行温室盆栽试验。分别在拔节期、孕穗期、抽穗期和灌浆期采集样本,利用凯氏定氮法测试叶片全氮含量,质谱仪测定^(15)N丰度。研究表明,不同生育期水稻叶片全氮含量和^(15)N丰度均随施氮水平逐渐增加。顶1叶片全氮含量和施肥量仅在拔节期极显著相关性,但是在全生育期3个叶位^(15)N丰度均与施氮量均达到0.01水平极显著相关。^(15)N示踪表明拔节期低氮素水平下位叶片^(15)N丰度较上位高;在高氮素水平上位叶片^(15)N丰度增加;孕穗期上位叶的氮素大量转移到穗部,导致孕穗期上位叶的^(15)N丰度明显小于下叶位。由于氮素营养的运转,抽穗期和灌浆期下叶位^(15)N丰度明显小于上叶位;叶片^(15)N丰度的变化更能反映出肥料供应的差异状况。叶片^(15)N丰度的变化更能反映出肥料供应的差异状况,和叶片全氮含量比较,叶片^(15)N丰度与施氮肥量的相关性更能说明施氮肥量和叶片氮素营养的关系。Based on the 15N tracer method,a series of greenhouse potted experiments were conducted with three rice cultivars(Lindao11,Shengdao13 and Yangguang200) to investigate the relationships between rates of fertilization and leaf nitrogen concentration(including 15N abundance and total nitrogen).The results showed that leaf nitrogen concentration and 15N abundance were sensitive to increased nitrogen supply.The relationships between leaf nitrogen concentrations and the rates of fertilization indicated that the 1st leaf total nitrogen concentrations significantly correlated with the rates of fertilization just at jointing stage.However 15N abundance of the 1st,2nd and 3rd leaf was significantly correlated with the rates of fertilization at 0.01 levels during all growth stages.15N tracer method showed that the 15N abundance in lower leaf were higher than that in upper leaf at the low rates of fertilization and 15N abundance increased at high rates of fertilization at jointing stage.But nitrogen transferred to the fringe from the 1st leaf,as result 15N abundance in upper leaf reduced at booting stage.With transfer of nitrogen from lower leaves to upper leaves at heading and milky stages,15N abundance in lower leaf were distinctively less than in the upper leaf.The 15N abundance change can reflect the difference condition of fertilizer supply.The correlationship between 15N abundance and nitrogenous fertilizer quantity was better to show relations between nitrogenous fertilizer quantity and nitrogen nutrition.15N tracer technique would be to achieve more desirable results in the diagnosis and recommended amount of nitrogen and nitrogen utilization of rice research.
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