葡萄幼苗对温度逆境交叉适应过程中HSP70在叶片中的亚细胞定位  

Sub Cellular Distribution of Heat Shock Protein 70 in Young Grape Leaves during Cross-adaptation to Temperature Stresses

作　　者：张俊环[1,2] 王玉柱[2] 孙浩元[2] 杨丽[2] 黄卫东[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]北京市农林科学院林业果树研究所,北京100093

出　　处：《中国农学通报》2010年第3期173-178,共6页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"高温诱导葡萄水杨酸信号的产生及其细胞分子识别"(30471192)资助项目

摘　　要：为了进一步分析热激蛋白在温度锻炼诱导的交叉适应性形成过程中的保护功能,此文以葡萄(Vitis vinifera L.cv.Jingxiu)幼苗为试材,采用胶体金免疫电镜定位技术,定位观察了HSP70在葡萄叶片中的亚细胞分布情况。结果表明,低温锻炼预处理可增强其在高温胁迫条件下的表达水平,细胞核和叶绿体中代表HSP70的免疫金颗粒密度比相应的对照细胞明显增多,尤其是胁迫处理3 h时。与低温锻炼相似,高温锻炼预处理增强了葡萄叶片在高温胁迫下细胞核和叶绿体中HSP70的表达量。这一结果为HSP70参与温度锻炼诱导的交叉适应性提供了更直观的细胞学证据。In order to investigate the protecting role of heat shock proteins in the cross-adaptation to temperature stresses induced by cold acclimation（CA） pretreatment or heat acclimation（HA） pretreatment. Leaves from two-years-old young grape（Vitis vinifera L.cv.Jingxiu） plants were used as experimental materials.The subcellular localizations of HSP70 within mesophyll cells were observed using immuno-gold labeling electron microscopic technique.The results showed that CA pretreatment could enhance the expression of HSP70 during heat stress.Compared to non-acclimated plants,the density of HSP70 gold particles was obviously increased in the nucleus and chloroplast of CA leaves,especially 3 h after heat stress. Similar to CA,HA pretreatment can protect the expression of HSP70 during cold stresses,and the density of HSP70 gold particles remains high level than that in control plants.These data offered the cytological evidence of HSP70 was contributed to the cross-adaptation to different temperature stresses induced by CA-,or HA-pretreatment in grape plants.

关 键 词：葡萄幼苗 交叉适应 温度逆境 HSP70 胶体金免疫电镜定位 

分 类 号：S663.1[农业科学—果树学]