中华鳖血液凝固特性研究  

A Study on the Characteristics of Blood Coagulation for Chinese Soft-shelled Turtle

作　　者：闫书彩[1] 李玉荣[1] 高明[1] 王韫[1] 曹栋[1] 李双安[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071000

出　　处：《中国农学通报》2010年第3期311-314,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金资助项目(30670260)

摘　　要：通过中华鳖全血、无细胞血浆(CFP)的凝固指标及水的稀释作用对这些指标的影响来阐述其凝血特性,指标包括全血复钙时间(CT)及血浆的复钙时间(RT)、活化凝血时间(ACT)、活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)、凝血酶时间(TT)6个方面。结果显示:试验首次报道鳖无细胞血浆的ACT、APTT和PT分别为332.65、147.75和50.61 s,与哺乳动物存在差异,但CT、RT和TT与哺乳动物没有差异;CFP复钙在10 min内未凝固,而CFP稀释后血浆凝固;CFP稀释后ACT、APTT与未稀释相比缩短(P<0.01),PT无变化(P>0.05)。结论:稀释后的鳖CFP发生凝固,主要通过影响内源性凝血过程,与外源性凝血途径无关。In order to discover the characteristics of blood coagulation for Chinese soft-shelled turtle,some general blood clotting tests have been done,for example,the whole blood clotting time（CT）test,the plasma recalcification time（RT） test,the activated plasma clotting time（ACT） test,the activated partial thromboplastin time（APTT） test,the prothrombin time（PT） test and the thrombin time（TT） test,the samples of whole blood or plasma were treated with citrate and differently diluted 1-,2-,3-fold with ion-free water.The results prove that：this paper firstly reported the ACT,the APTT and the PT for Chinese soft-shelled turtle were 332.65, 147.75,50.61 s,which are different from mammals,however,the CT,the CT and the TT are no difference between Chinese soft-shelled turtle and mammals;the cell-free plasma of Chinese soft-shelled turtle uncoagulates within 10 min.,but the diluted cell-free plasma of Chinese soft-shelled turtle coagulate;the ACT and the APTT are extremely significant difference（P〈0.01） between the diluted plasma and the control group for Chinese soft-shelled turtle,however,the PT is nonsignificant difference（P0〉.05） between the diluted plasma（1：1,1：2） and that of the control group.Conclusions：the diluted cell-free plasma of Chinese soft-shelled turtle has coagulated,primarily by influencing the intrinsic pathway but has nothing to do with the extrinsic pathway.

关 键 词：中华鳖 血液 凝固 

分 类 号：S917[农业科学—水产科学]