联合抗体检测诊断原发性干燥综合征  

Application of combined antibodies in the diagnosis of primary Sjgren's syndrome

作　　者：常新[1] 丁翔[1] 任义乐[1] 

机构地区：[1]连云港市第一人民医院风湿免疫科,江苏省222000

出　　处：《中华实用诊断与治疗杂志》2010年第3期221-223,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：江苏省连云港市卫生局课题(08039)

摘　　要：目的:探讨抗SSA抗体、抗SSB抗体、抗α-fodrin蛋白抗体联合检测在原发性干燥综合征诊断中的应用价值。方法:原发性干燥综合征患者42例(观察组),健康体检者77例(对照组),应用斑点法检测抗SSA抗体、抗SSB抗体,ELISA法检测抗α-fodrin蛋白抗体。结果:抗SSA抗体、抗SSB抗体、抗α-fodrin蛋白抗体在观察组中的阳性率分别为71.4%、40.5%、33.3%,对照组的阳性率分别为15.6%、3.9%、7.8%。抗SSA抗体、抗SSB抗体、抗α-fodrin蛋白抗体在观察组中的特异性分别为84.4%、96.1%、92.2%。观察组抗SSA抗体和抗SSB抗体均阴性者,抗α-fodrin蛋白抗体的阳性率达41.7%。抗SSA抗体、SSB抗体联合抗α-fodrin蛋白抗体检测,可使原发性干燥综合征诊断的阳性率提高至83.3%。结论:抗SSA抗体、抗SSB抗体联合抗α-fodrin蛋白抗体检测,可提高原发性干燥综合征诊断的敏感性。抗SSB抗体及抗α-fodrin蛋白抗体在原发性干燥综合征诊断中均有较高特异性。Objective To study the significance of combination of anti-α-fodrin antibody, anti-SSA antibody and anti-SSB antibody in the diagnosis of primary Sjogren＇s syndrome. Methods The anti-α- fodrin antibody was detected with spotting method, and anti SSA antibody and anti-SSB antibody were detected with ELISA in 42 patients with primary Sjogren＇s syndrome（observation group） and 77 healthy controls（control group）. Results The positive rates of anti-SSA antibody, anti-SSB antibody and anti-α-fodrin antibody were 71. 4%, 40. 5% and 33. 3% in the observation group, and 15. 6%, 3.9% and 7. 8% in control group. The specificities were 84. 4%, 96. 1% and 92. 2% in the observation group. The anti-SSA antibody and anti SSB antibody were negative in the observation group and positive rate of anti-α-fodrin antibody was 41.7%. The sensitivity of the combination of the three markers was up to 83. 3%. Conclusion The combination of anti-α-fodrin antibody, anti-SSA antibody and anti-SSB antibody can improve the sensitivity for the diagnosis of primary Sjogren＇s syndrome. Anti-α-fodrin antibody and anti SSB antibody have high specificities in the diagnosis of primary Sjogren＇s syndrome.

关 键 词：原发性干燥综合征 抗α-fodrin蛋白抗体 自身抗体 

分 类 号：R593.2[医药卫生—内科学]