H7N7亚型禽流感病毒血凝素B细胞和Th细胞表位预测及初步鉴定  

Prediction of the B cell/Th Cell Epitopes on HA of Avian in Fluenza Virus(H7N7)and Preliminary Evaluation.

作　　者：白靓[1] 金宁一[2] 成岩[1] 石毅[1] 鲁会军[2] 南文龙[2] 田明尧[2] 赵翠青[2] 张金双[2] 关铭[1] 

机构地区：[1]吉林大学药学院,长春130021 [2]军事医学科学院11所全军基因工程重点实验室,长春130062

出　　处：《中国农学通报》2010年第4期11-14,共4页Chinese Agricultural Science Bulletin

基　　金：国家高技术研究发展(863)计划"家禽重要病毒病基因工程疫苗研究和创新"(NO:2006AA10A205)

摘　　要：预测H7N7亚型禽流感病毒血凝素(Hemagglutinin,HA)B细胞和Th细胞(THelpCell,辅助T细胞,在免疫调节中起促进作用的T细胞亚群。)相关抗原表位,并对所获表位的抗原性进行初步鉴定。根据禽流感流行趋势,从GeneBank下载H7N7禽流感病毒HA氨基酸序列,使用生物信息学方法,对所下载序列进行预测与分析,获得H7N7亚型禽流感病毒血凝素B细胞和Th细胞相关抗原表位。通过H7亚型禽流感病毒阳性血清,初步验证所选表位抗原性。获得了5个H7N7亚型禽流感病毒候选B细胞和Th细胞表位,经过间接ELISA方法分析,其中HA165～178,HA180～193,HA241～255表序列相对保守,与流行的H7N7亚型禽流感病毒HA相应区域具有较好的一致性,同时具有与H7型禽流感病毒阳性血清抗体结合能力,预示了其成为功能表位的可能。所筛选的表位具有成为H7N7亚型禽流感病毒HAB细胞和Th细胞相关抗原表位的可能,为H7N7亚型禽流感表位疫苗的研究奠定基础。To predict B/Th cell epitopes on Hemagglutinin（HA）of avian influenza virus（H7N7）and evaluate antigenicity of the candidate epitopes.The HA amino acid sequences of AIV（H7N7）which were prevalent recently,were downloaded from GeneBank.The Th/B cell epitopes were predicted and analyzed by bioinformatics methods.Then,antigenicity of the candidate epitopes was identified by AIV（H7）positive serum samples.Five B/Th cell epitopes containing HA were acquired.HA165~178,HA180~193,HA241~255epitopes which were analyzed by indirect ELISA method were on a relatively conserved domain and a deal of AIV（H7N7）contained the sequences.Moreover,the candidate epitopes were showed with a distinct antibody combining reactivity with the H7 HA positive serum,which inferred the predicted epitopes to be functional ones.The selected epitopes are able to be functional HA B/Th cell epitopes of AIV（H7N7）.Our study also establishes the foundations for the recognition of AIV（H7N7）functional epitopes and the development of epitope vaccines.

关 键 词：禽流感病毒 血凝素 H7N7 抗原表位 

分 类 号：S859.797[农业科学—临床兽医学]