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作 者:易晓华[1,2]
机构地区:[1]西北农林科技大学无公害农药研究服务中心/陕西省生物农药工程技术研究中心,陕西杨凌712100 [2]青岛农业大学生命科学学院,山东青岛266109
出 处:《中国农学通报》2010年第4期249-253,共5页Chinese Agricultural Science Bulletin
基 金:国家"十五"科技攻关重大专项资助项目(2002BA516A04)
摘 要:从除虫菊叶中分离到1株内生镰孢菌Y2,环境和营养因子对该菌的生长繁殖和抑菌活性影响较4大.0:~在9.54~时40,菌℃丝时生,菌长丝、孢能子生形长成并和形萌成发孢均子良,最好适,最生适长繁pH殖为温5度.5。为氮30源℃以,有孢机子态的氮致为死佳温。度分为别60以℃D。-木在糖pH,山梨糖,甘露醇为碳源,以硝酸钠、甘氨酸、硫酸铵、牛肉膏和蛋白胨为氮源培养时,该菌株发酵液抑菌活性较高。An endophytic fungus Fusarium sp.Y2 found from the leaves of Pyrethrum cinerariifolium Trev.,the effect of environmental and nutritional factors on its growth and reproduction was remarkable.The temperature from 4℃to 40℃were suitable for mycelial growth,sporulations and germination.The optimum temperature was 30℃for sporulation,the lethal temperature was 60℃for 10 minutes.pH value ranged from 4.0 to 9.5 was favourable for mycelial growth,sporulation and spore germination.The optimum pH was 7.5.As to nitrogen sources,the fungus utilized organic nitrogen very well.Taking D-xylose,sorbitol or mannitol as carbon source,with glycine,ammonium sulfate,beef extract or peptone as nitrogen source,the antifungal activity of the fungal fermenting liquid was comparatively high.
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