广玉兰叶乙醇提取物对植物病原真菌抑菌活性研究  

Antifungal Activity of Ethanol Extract from Magnolia grandiflora

作　　者：程永芳[1] 宗磊[1] 赵莉[1] 金玉兰[1] 

机构地区：[1]青岛农业大学化学与药学院,山东青岛266109

出　　处：《中国农学通报》2010年第4期267-270,共4页Chinese Agricultural Science Bulletin

基　　金：青岛农业大学人才基金(630437)

摘　　要：同过测定广玉兰叶的乙醇提取物以及不同溶剂萃取层对植物病原真菌的抑菌活性,寻找广玉兰叶中的农用活性物质,为其综合开发利用提供科学依据。采用菌丝生长速率法测定了广玉兰叶乙醇提取物对8种植物病原菌的抑制作用。结果表明,当供试浓度为1.0mg/mL时对苹果腐烂病菌(Valsa mali)、白菜黑斑病菌(Alternaria brassicicola)的抑菌率比较高,抑菌率分别为81.4%和52.1%。当供试浓度为5.0mg/mL时,对小麦赤霉病菌(Gibberella zeae)、棉花枯萎病菌(Fusarium oxysporum f.sp.vasinfectum)和苹果轮纹病菌(Physalospora piricola nose)的抑菌率也比较高,均为50.0%以上。乙醇提取物对苹果腐烂病菌和白菜黑斑病菌EC50分别为0.04mg/mL和1.20mg/mL。广玉兰叶乙醇提取物的不同极性溶剂萃取物中正己烷层与乙酸乙酯层的抑菌活性较高,EC50分别为0.03mg/mL和0.11mg/mL。活性物质主要集中于正己烷和乙酸乙酯萃取部分,是进一步研究的重点。In order to discover the agricultural activity and fungicidal ingredient in the leaves of Magnolia grandiflora Lima and provide scientific proofs of its exploitation and utilization,antifungal activity of the extracts from leaves of M.grandiflora.Lima with ethanol and different solvents were studied.Using a mycelial growth method,the fungistasis of the extracts with ethanol against eight kind of phytopathogenic fungi were investigated in this study.The results showed that the extracts have strong antifungal activity against the tested pathogenic fungi.The inhibition rate was 81.4%and 52.1%against Valsa mali and Alternaria brassicicola,respectively,at 1.0 mg/mL.The inhibition rate was above 50%against Gibberella zeae,Fusarium oxysporum f.sp.vasinfectum and Physalospora piricola nose at 5.0 mg/mL.The EC50of ethanol extracts against V.mali and A.brassicicola were 0.04 and 1.20 mg/mL,respectively.The ethanolic extract was fractionated using solvents of increasing polarity,the n-hexane and ethyl acetate fraction had stronger antifungal activities than that of extracts with solvents n-butanol and water.The EC50of n-hexane and ethyl acetate fraction against V.mali was 0.03 and 0.11 mg/mL,respectively.These indicated that most of the activity components were distributed in the n-hexane and ethyl acetate fraction with would be further studied as the emphases.

关 键 词：广玉兰 植物病原真菌 抑菌活性 苹果腐烂病菌 白菜黑斑病菌 

分 类 号：S482.292[农业科学—农药学]