一例特异细胞质雄性不育小麦mtDNA特异片段的克隆和测序  

CLONING AND SEQUENCING OF mtDNA SPECIAL FRAGMENTS OF A SPECIAL CMS IN WHEAT

作　　者：孙瑞[1] 张改生[1] 牛娜[1] 马守才[1] 李红霞[1] 汪奎[1] 袁正杰[1] 张龙雨[1] 

机构地区：[1]西北农林科技大学陕西省作物杂种优势研究与利用重点实验室/小麦育种教育部工程研究中心,陕西杨陵712100

出　　处：《核农学报》2010年第1期12-19,共8页Journal of Nuclear Agricultural Sciences

基　　金：国家高技术研究发展计划(863计划)重大专项(2009AA101102);陕西省"13115"科技创新工程重大科技专项(No.2007ZDKG-020);西北农林科技大学拔尖人才支持计划项目

摘　　要：以具有同质同核的二角型小麦花药外露不育系与花药不外露突变不育系为试材,对其mtDNA进行了RAPD分析,筛选出特异片段S32-1582、OPAA16-1753,并进行克隆与测序。结果表明:花药外露型不育系与花药不外露型不育系细胞质内mtDNA存在明显差异,而ctDNA之间不存在差异;二角型花药外露型不育系转化为花药不外露型不育系,很可能是由于mtDNA自发的重排引起的。其差异片段S32-1582、OPAA16-1753序列与核基因组上的序列具有同源性;供试线粒体基因组能够在较短的演化时间内发生变异,从而引起二角型花药外露型不育系向花药不外露型不育系的变异,其特异质核互作可能起重要作用。The mitochondrial DNA（mtDNA） were analyzed between two male sterile lines,anther outside floret and anther inside floret with same Ae.bicornis cytoplasm in wheat by RAPD technique,and two specific bands S32-1582 and OPAA16-1753 were screened,then cloned and sequenced.The results showed that the difference was found between mitochondrial DNA（mtDNA） of two male sterile lines,but there was no difference in their chloroplast DNA（ctDNA）.Transfering the male sterile line of anther outside floret to anther inside floret line revealed that it was most likely due to the autonomic rearrangement of mtDNA.The sequences of S32-1582 and OPAA16-1753 were homologous with the nuclear genome sequence.The results indicated that the test plant mitochondrial genome mutated in a relatively short period of time,thus caused bicornis sterility with anther outside floret to anther inside floret,the specific nuclear-cytoplasmic interaction probably might play an important role in the process.

关 键 词：小麦 二角型不育 MTDNA RAPD 

分 类 号：S512.1[农业科学—作物学]