蛋氨酸对山羊内源氮和氨基酸流量影响的测定方法比较  

COMPARISON OF DETERMING METHODS FOR METHIONINE EFFECT ON ENDOGENOUS FLOWS OF NITROGEN AND AMINO ACIDS IN GROWING GOATS

作　　者：周传社[1] 王久荣[1] 潘亚非[1] 谭支良[1] 汤少勋[1] 孙志洪[1] 韩雪峰[1] 王敏[1] 

机构地区：[1]中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,湖南长沙410125

出　　处：《核农学报》2010年第1期181-186,72,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家“十一五科技支撑”课题(2008BADA7B04)

摘　　要：采用3×3拉丁方试验设计,选用3头安装有永久性瘤胃、十二指肠近端瘘管和颈静脉插管,平均体重为20kg±2.5kg的健康湘东黑山羊为试验动物,饲喂Met水平分别为0.15%、0.25%和0.35%的3种试验日粮,旨在研究Met水平对山羊内源氮(EN)和内源氨基酸(ENAA)流量的影响,同时比较差量法和同位素稀释法测定山羊十二指肠EN和ENAA流量的差异。结果显示:日粮Met水平对山羊十二指肠EN和ENAA流量没有显著影响(P>0.05);当日粮Met水平为0.15%时,差量法和同位素稀释法测定十二指肠EN和ENAA流量均最低,且2种测定方法之间无显著差异(P>0.05);差量法测定EN和ENAA流量均低于同位素稀释法测定结果(P>0.05)。结果表明:山羊十二指肠EN和ENAA流量不受日粮Met水平影响;差量法测定的十二指肠EN和ENAA流量低于同位素稀释法。To compare the results gained from the difference method and isotope dilution method by feeding normal protein-containing diets,the effects of dietary methionine（Met） levels on the duodenal flows of endogenous N（EN） and endogenous amino acids（ENAA） in growing goats were studied.Three tested goats（20kg ± 2.5kg） were fitted with a ruminal,duodenal cannulae and the jugular catheters,and allocation to three dietary treatments using 3 × 3 Latin Square design.The dietary treatments consisted of a total mixed ration containing three levels of Met（0.15%,0.25% and 0.35%）,respectively.The results showed that the duodenal flows of EN and ENAA was the lowest when the dietary Met level was 0.15%,and there was no significant difference（P0.05） in the duodenal flows of EN and ENAA measured by the difference method and isotope dilution method.The duodenal flows of EN and ENAA determined by the difference method was lower than the data by the isotope dilution method（P0.05）.It was concluded that the duodenal flows of EN and ENAA are not affected by the dietary direct supplementation of Met,and the duodenal flows of EN and ENAA determined by the difference method were lower than data by the isotope dilution method.

关 键 词：蛋氨酸 差量法 同位素稀释法 内源氮 内源氨基酸 流通量 山羊 

分 类 号：S827[农业科学—畜牧学]