涡流色谱技术在生物样品分析中的应用  

Application of turbulent flow chromatography in the analysis of biological samples

作　　者：刘朋[1] 周建良[1] 安婧婧[1] 李萍[1] 

机构地区：[1]现代中药教育部重点实验室中国药科大学生药学教研室,江苏南京210009

出　　处：《色谱》2010年第2期168-174,共7页Chinese Journal of Chromatography

基　　金："十一五"国家"重大新药创制"科技重大专项(No.2009ZX09502-020);中国药科大学研究生创新计划项目(No.02704006-522)

摘　　要：生物样品的复杂性使其在进行分析测定前必须经过处理。传统的样品前处理方法(如液-液萃取、固相萃取等)耗时长且操作繁琐。涡流色谱作为在线萃取技术,可以实现生物样品直接进样,减少了样品处理步骤,有效富集纯化了分析物,是一种高通量、高选择性的生物样品前处理方法。为此,本文介绍了涡流色谱技术的原理及优势,并总结了不同涡流柱的特点及其在生物样品分析领域中的应用情况。The complexity of biological fluids makes the sample preparation necessary in the analytical process.The traditional techniques,such as liquid-liquid extraction and solid-phase extraction,are time-consuming and labor-intensive.Turbulent flow chromatography（TFC）,as an on-line direct injection technique,possesses the advantages of reducing sample preparation steps and enabling the effective pre-concentration and clean-up of biological fluids.Therefore,it is a high-throughput and high-selectivity technique for biological sample pretreatment.In this review,the theory,advantages,characteristics and current status of TFC in bioanalysis are introduced and summarized.

关 键 词：涡流色谱 样品前处理 生物样品 

分 类 号：O658[理学—分析化学]