利用野生稻选育测253、测258恢复系及测交组合分子标记检测  

Molecular Marker Detection of Restoring Line Ce 253,Ce 258 Bred by Wild Rice and Their Test Cross Combinations

作　　者：莫永生[1] 黄娟[2] 梁云涛[2] 陈成斌[2] 蔡中全[1] 

机构地区：[1]广西大学支农开发中心,广西南宁530005 [2]广西农业科学院水稻研究所,广西南宁530007

出　　处：《江西农业学报》2010年第1期8-9,16,共3页Acta Agriculturae Jiangxi

基　　金：广西科技攻关计划项目(桂科攻0012027;0228002)

摘　　要：以不同野生稻和栽培稻亲本及其杂交后代恢复系测253、测258和测交组合博优253、博优258为材料,利用水稻12条染色体上的120对引物和SSR标记检测野生稻、栽培稻亲本在杂交后代中的遗传表现。结果表明,在120对引物中,6对引物RM28、RM287、RM347、RM303、RM278、RM154的多态性效果较好;6对SSR引物的扩增产物显示,野生稻的DNA片段已被导入野栽型恢复系及其优良组合中,并且分布在不同染色体上,说明通过远缘杂交大规模导入野生稻细胞核基因,对拓宽恢复系遗传背景是一个有效的方法。The present study was conducted to detect the genetic expression of wild rice and cultivated rice parents in their hybridization progenies. Different wild rice （Tiandong, Tianyang, Tianlin wild rice）, cultivated rice （IR36, IR24） parents and their hybridization progeny restoring lines （Ce 253, Ce 258） and test cross combinations （Boyou 253, Boyou 258） were used as materials and detected by 120 pairs of primers and SSR markers. It was found that among 120 pairs of primers, 6 pairs of primers, viz. , RM28, RM287, RM347, RM303, RM278 and RM154 showed better effects on polymorphism of these rice materials. The amplification prod- ucts of 6 pairs of SSR primers showed that the DNA fragments of wild rice had been introduced into restoring lines Ce 253, Ce 258 and test cross combinations Boyou 253, Boyou 258, and these fragments were located in different chromosomes. These results indicated that the introduction of nucleus genes from wild rice by distant hybridization would be an effective method to widen the genetic background of restoring line.

关 键 词：野生稻 恢复系 测交 SSR检测 

分 类 号：S511.9[农业科学—作物学] S511.035.1