固始-安卡鸡F_2资源群△-9脂肪酸脱氢酶基因第4、5、6外显子SNP及其与脂肪酸相关性研究  

Study on SNP of △-9 Desaturase Gene Extron 4,5,6 and Its Correlation with Some Fatty Acids in F_2 Chickens Derived from Gushi-Anka Chick

作　　者：刘凯[1] 张立恒[1] 康相涛[1,2] 韩瑞丽[1] 王润之[1] 马新红[1] 亢娟[1] 黄煌[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]河南省家禽种质资源工程研究中心,河南郑州450002

出　　处：《江西农业学报》2010年第1期143-147,共5页Acta Agriculturae Jiangxi

基　　金：国家科技支撑计划(2008BADB2B07-5);国家公益性行业(农业)科研专项(nyhyzx07-039);国家蛋鸡产业技术体系项目资助;国家农业科技成果转化资金项目(2009D00103);河南省杰出人才创新基金(074200510009)

摘　　要：利用PCR-SSCP(single strand conformation polymorphism)方法,对固始-安卡鸡F2代资源群△-9脂肪酸脱氢酶基因第4、5、6对外显子多态性进行分析,发现存在一个单核苷酸多态(SNP,single nucleotide polymorphisms)位点。在△-9脂肪酸脱氢酶基因第4外显子编码区(89nt)发生了碱基的转换突变(G→A)。在整个F2代资源群中进行了该多态位点与脂肪酸性状的相关分析,结果表明:在8个脂肪酸性状中,AA型个体十六碳二烯酸(C16∶2)含量显著高于GG型和GA型个体(P<0.05);家系1中AA型的C16∶0、C16∶2、C18∶0含量均显著高于GG型和GA型(P<0.05);家系3中AA型的C14∶0含量显著高于GG型和GA型(P<0.05)。PCR - SSCP technology was used to analyze the polymorphisms of extron 4, 5, 6 of △- 9 desaturase gene in F2 chick- ens derived from Gushi chick to Anka chick. A SNP locus and G--A mutation at 89 nt of the extron 4 were found. The correlation statistical analysis indicated that F2 chickens with genotype AA had significantly higher C16：2 content （P 〈 0. 05 ）. Family 1 chickens with genotype AA had significantly higher C16： 0, C16： 2, C18：0 content （ P 〈 0. 05 ）. Family 3 chickens with genotype AA had signif- icantly higher C14：0 content （P 〈0.05）.

关 键 词：鸡 Δ-9脂肪酸脱氢酶基因 单核苷酸多态性 PCR-SSCP 脂肪酸 

分 类 号：S831[农业科学—畜牧学]