激光扫描共聚焦显微技术及其在神经、肿瘤相关研究中的应用  

Laser Scanning Confocal Microscope Technology and Its Application in Neurology and Tumor Researches

作　　者：张鹏[1] 毕明刚[2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心药物研究所博士后科研工作站,哈尔滨150076 [2]中国医学科学院药用植物研究所

出　　处：《医疗卫生装备》2010年第2期43-45,49,共4页Chinese Medical Equipment Journal

基　　金：国家自然科学基金项目(30873090)

摘　　要：介绍了近年来新出现的共聚焦显微镜新的种类,如传统激光共聚焦和活细胞激光共聚焦,对其功能及在神经、肿瘤研究中的应用进行了综述,使现代显微镜能够更加深入研究和分析细胞的变化过程和结构。活细胞激光扫描共聚焦显微镜与双光子激光扫描共聚焦显微镜实现了对肿瘤、神经活细胞长时间地动态观测,可更加真实地揭示细胞凋亡的机制与规律。Newly developed confocal microscopes, such as traditional laser confocal microscope and living cell confcal mi- croscope, are introduced and their functions and application in researches on neurology and tumor are reviewed. Modern microscopes can be used to study the change process and structure of cells. Long-time dynamic observation of living tumor and nerve cells is performed by living cell laser scanning eonfoeal microscope and two-photon laser scanning eonfocal microscope, and the mechanism and rule of apoptosis can be disclosed. [Chinese Medical Equipment Journal,2010,31 （2） ：43-45,49]

关 键 词：激光扫描共聚焦 基因敲除 活细胞检测 凋亡 

分 类 号：R318.51[医药卫生—生物医学工程]