川芎嗪对早期糖尿病大鼠胸主动脉BKCa通道的影响  

Tetramethylpyrazine effects on aortic vascular cellular BKCa channel of early stage diabetic rats diabetic

作　　者：农慧[1] 盛庆寿[2] 丁胜元[3] 闫福曼[3] 罗荣敬[3] 

机构地区：[1]广西中医学院生理教研室,南宁530001 [2]广西中医学院附属瑞康医院,南宁530011 [3]广州中医药大学生理教研室,广州510405

出　　处：《中药药理与临床》2009年第6期36-39,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：广州中医药大学中医药科研究创新基金资助项目;项目号:2005C01

摘　　要：目的:研究川芎嗪对早期糖尿病大鼠胸主动脉BKCa通道影响,从离子通道的角度探讨川芎嗪治疗糖尿病血管并发症的可能机制。方法:用链脲佐菌素大剂量单次腹腔注射法造成大鼠糖尿病模型,大鼠随机分为4组:正常组、糖尿病模型组、川芎嗪低剂量组和川芎嗪高剂量组。采用腹腔注射给药,正常组和模型组腹腔给予射注射用水,川芎嗪低、高剂量组分别按40mg/kg、80mg/kg给药,用药8周后,处死大鼠。酶分离法获取单个胸主动脉平滑肌细胞,单通道膜片钳技术记录BKCa电流,计算平均通道开放概率。结果:糖尿病模型组、川芎嗪低、高剂量组的BKCa通道平均开放概率分别是0.20±0.12、0.31±0.24、0.40±0.19高于正常组0.09±0.02(P<0.01),川芎嗪组高于模型组(P<0.01),川芎嗪高剂量组高于低剂量组(P<0.05)。结论:糖尿病早期胸主动脉的BKCa通道会代偿性的增加开放概率;川芎嗪可提高早期糖尿病大鼠胸主动脉BKCa通道的活性,增加其平均开放概率,可能是其治疗糖尿病血管病变的机制之一。Objective：To study tetramethylpyrazine（TMP） effected on large conductance calcium-activated potassium channel（BKCa channel）,then find out the possibile mechanism of TMP in curing diabetic angiopathy diseases.Methods：One large dose streptozocine intraperitoneally injected to establish diabetic rats model.All rats were divided into four groups randomly,they were normal group,DM model group,TMP low dose group,TMP high dose group.Rats in nornal group and DM model group were intraperitioneally injected with water for injection;in TMP low dose and high dose groups,were intraperitoneally injected with TMP injection 40mg/kg,80mg/kg respectly,all once a day.8 weeks later,the all rats were killed one after another,arota thoracica ioslated were digested in enzyme solution to get single VSMC for cell attached patch-clamp technique test,a method used to record BKCa current amplitude,open posssibality（NPO）,time of open（TO）,time of close（TC）.Results：The NPO were 0.20±0.12、0.31±0.24、0.40±0.19,in diabetic model group,TMP low dose group,TMP high dose group,respectly,increased significantly compared with normal group 0.09±0.02（P0.01）.The NPO of TMP high dose group was higer than TMP low dose group（P0.01）,the later was higher than DM model group（P0.01）.Conclusion：The study observed that the NPO of BKCa on VSMCs was increased as compensation,TMP could enhance the NPO,in the early phase of diabetes rats.

关 键 词：川芎嗪 糖尿病 BKCA通道 

分 类 号：R285.5[医药卫生—中药学]