HO-1活性变化对HIF-1基因表达的影响  

Effect of heme oxygenase 1 on gene expression of hypoxia inducible factor 1

作　　者：刘立青[1] 张黎[1] 程力[1] 陈洪元[1] 苗瑞政[1] 

机构地区：[1]山东大学附属省立医院胃肠外科,山东济南250021

出　　处：《中国现代普通外科进展》2009年第12期1023-1025,1099,共4页Chinese Journal of Current Advances in General Surgery

基　　金：山东省自然科学基金项目(Y2007C127)

摘　　要：目的:探讨血红素氧化酶1(HO-1)活性变化对缺氧诱导因子1(HIF-1)基因表达的影响及其可能机制。方法:利用乏氧培养箱建立胃癌细胞株的缺氧诱导模型,采用RNA干扰技术使HO-1基因沉默,设为转染组(Z组);利用血晶素(Hemin)诱导使HO-1活性升高,设为Hemin组(H组);对照组(D组)仅做乏氧培养。分别用RT-PCR和免疫组织化学技术测定各组HO-1和HIF-1的mRNA及蛋白质含量。结果:Z组HO-1和HIF-1的mRNA和蛋白质水平明显低于D组(P<0.01),而H组明显高于D组(P<0.01)。结论:HO-1在接受HIF-1调节的同时,对HIF-1有正反馈的作用。这为今后利用RNA干扰等基因技术治疗恶性肿瘤提供了实验依据和理论基础。Objective： To investigate the effect of heme oxygenase-1 on gene expression of hypoxia inducible factor-1 and the possible mechanism. Methods： Hypoxia-inducible models of gastric cancer cell lines were set up. Three groups were assigned： Group Z,using RNA interference technology to suppress the gene expression of HO-1 to reduce its activity; Group H,hemin was used to promote the expression of HO-1; Group D,as control group. RT-PCR technique was used in each group to determine mRNA levels of HO-1 and HIF-1; Immunohistochemical technique was used in each group to detect protein levels of HO-1 and HIF-1. Results： The mRNA and protein level of HO-1 and HIF-1 in Group Z was significantly lower than that in the control group; Whereas,in Group H, the protein and mRNA level of HO-1 and HIF-1was significantly higher than that in the control group. Conclusions： The up-regulation of HO-1 can promote the expressions of HIF1 in turn, suppressing the expression of HO-1 can reduce the activity of HIF1.

关 键 词：血红素氧化酶1·缺氧诱导因子1·Hemin.RNA干扰 

分 类 号：R735.2[医药卫生—肿瘤]