芪芍五味子复方制剂抗CVB3病毒抑制心肌细胞凋亡机制的研究  

Antiviral effect and inhibition of cardiomyocyte apoptosis of the Qishaowuweizi compound

作　　者：刘奉琴[1] 王玉林[1] 靳有鹏[1] 朱晓新[2] 王青青[1] 宋嘉[1] 

机构地区：[1]山东大学附属省立医院儿科,济南250021 [2]中国中医研究院中药研究所,北京100091

出　　处：《山东大学学报（医学版）》2010年第2期33-37,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(30672710);教育部高等学校博士学科点专项科研基金(20060422047);山东省自然科学基金(Y2003C02)

摘　　要：目的研究中药芪芍五味子复方制剂抗柯萨奇B3病毒(CVB3)效果及抑制病毒性心肌炎小鼠心肌细胞凋亡的机制。方法采用细胞培养法观察细胞病变效应(CPE)与广谱抗病毒药物病毒唑对照,进行复方制剂体外抗病毒实验;BALB/c小鼠接种CVB3建立病毒性心肌炎动物模型,随机分为正常对照组、病毒对照组、复方制剂治疗组和维生素C与病毒唑联用组,观察各组心肌病理变化,Real-time定量PCR检测小鼠心肌CVB3RNA水平,流式细胞仪检测心肌细胞凋亡和坏死率。结果复方制剂体外最大无毒浓度为19.53g/L,预防给药、同时给药和治疗给药3种方式细胞培养所需有效药物浓度均低于病毒唑;与同期病毒对照组小鼠比较,复方制剂治疗组小鼠心肌炎症性病理变化明显减轻,CVB3RNA拷贝数降低(P<0.05),心肌细胞凋亡和坏死率显著降低(P<0.05)。结论芪芍五味子复方制剂可有效抗病毒,抑制病毒诱导心肌细胞凋亡,对CVB3感染小鼠心肌有良好的保护作用。Objective To investigate the antiviral effect against coxsackievirus B3 （ CVB3 ） and inhibition of cardiomyocyte apoptosis of the Traditional Chinese herb--Qishaowuweizi compound （QSW）. Methods The cytopathic effect （CPE） was observed in an in vitro antiviral experiment, with Ribavirin, a broad-spectrum antiviral drug, as the control. Male BALB/c mice were randomly divided into the virus control group, the QSW-treatment group and the Vit C plus ribavirin treatment group. The mice were infected with CVB3 to prepare a viral myocarditis model. They were sacrificed on experimental days 7, 14 and 21. The cardiac pathologic changes were checked by a light microscope and CVB3 RNA copy numbers by real-time quantitative PCR. Cardiomyocyte apoptosis and necrosis were detected by flow-cytometry （FCM）. Results The maximum atoxic concentration of QSW in vitro was 19. 53g/L. The CPE was more effectively at- tenuated by QSW than by Ribavirin in different administration groups in vitro. Compared with the virus control group, the QSW-treatment group had alleviated cardiac pathologic changes, decreased CVB3 RNA copy numbers, and lower cardiomyocyte apoptosis and necrosis ratios （all P 〈 0. 05 ）. Conclusion The qishaowuweizi compound has heart-protective effect in treating viral myocarditis through inhibiting cardiomyocyte apoptosis.

关 键 词：病毒性心肌炎 柯萨奇病毒B3 中药 细胞凋亡 

分 类 号：R725.4[医药卫生—儿科]