躯干神经嵴干细胞与壳聚糖纤维组织相容性的研究  

Histocompatibility of chitosan fiber and trunk neural crest stem cells

作　　者：杨洁[1] 孙晋浩[1] 张静[1] 卢静[1] 房芃[1] 杨淑华[1] 李振华[1] 

机构地区：[1]山东大学医学院解剖学教研室,济南250012

出　　处：《山东大学学报（医学版）》2010年第2期48-51,57,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助项目(Y20070031)

摘　　要：目的探讨体外培养的躯干神经嵴干细胞与壳聚糖纤维的相容性。方法采用神经管植块法培养躯干神经嵴干细胞,并行免疫荧光细胞化学双重染色鉴定,将培养48 h的躯干神经嵴干细胞接种在壳聚糖纤维材料上,观察两者的组织相容性。结果采用神经管植块法培养的神经嵴干细胞,免疫细胞化学染色呈现nestin及P75双阳性,将其接种在壳聚糖纤维支架材料上,可见神经嵴干细胞贴附于壳聚糖纤维上生长;S-100免疫细胞化学染色显示,S-100阳性细胞突起的末端呈扁平状膨大;扫描电镜显示,壳聚糖纤维上的躯干神经嵴干细胞为梭形,呈现"端对端"、"肩并肩"排列,伸出爪形伪足贴附于壳聚糖纤维上。结论躯干神经嵴干细胞与壳聚糖纤维有良好的组织相容性,并可分化为Schwann细胞。Objective To explore the histocompatibility of chitosan fiber with trunk neural crest stem cells in vitro. Methods Trunk neural crest stem cells were cultured on chitosan fiber using tissue culture of the neural tube in vitro. Immunofluoresecence was used to identify the trunk neural crest cells and observed histocompatibility. Results Trunk neural crest stem cells were successfully isolated and cultured. The immuocytochemcal result showed that the cells were nestin-and P75-positive. Trunk neural crest stem cells grew through adhering to chitosan fiber after inoculation. Under an electon microscope, the spindle-shaped trunk neural crest stem cells were proliferated and migrated along the fiber. The cells arranged side by side or linked by heads and tails in processes. The ends of S-100 positive cells were flat and expanded, adhering to the fiber as claw-shaped pseudopodium. Conclusion There is good histocompatibibity between chitosan fiber and trunk neural crest stem cells, and the later can differentiate into Schwann cells.

关 键 词：干细胞 细胞 培养的 组织相容性 壳聚糖纤维 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]