Th17T淋巴细胞相关细胞因子在免疫性前列腺炎组织中的表达  

Expressions of Th17 lymphocytes related cytokines in experimental autoimmune prostatitis tissue

作　　者：周玉海[1] 赵升田[1] 袁明振[1] 张旭峰[1] 陈友根[1] 黄生亮[1] 

机构地区：[1]山东大学第二医院泌尿外科,济南250033

出　　处：《山东大学学报（医学版）》2010年第2期63-66,共4页Journal of Shandong University:Health Sciences

基　　金：山东省中青年科学家科研奖励基金资助课题(2008BS03037)

摘　　要：目的探讨Th17辅助性T淋巴细胞相关细胞因子白细胞介素17A(interleukin17A,IL17A)、白细胞介素23(p19/p40)(interleukin23,IL23p19/p40)等在小鼠免疫性前列腺炎组织内的表达。方法应用Wistar大鼠前列腺组织制作前列腺蛋白提纯液,联合弗氏完全佐剂及百白破疫苗,多点皮下注射,免疫C57BL/6小鼠制作免疫性前列腺炎小鼠模型,对小鼠前列腺组织行HE染色,检测小鼠成炎情况;采用SYBR GreenⅠ一步嵌合荧光法实时定量RT-PCR检测免疫性前列腺炎组织Th17辅助性T淋巴细胞相关细胞因子IL17A、IL23p19、IL23p40等mRNA的表达,并与对照组比较上述细胞因子表达的区别。结果成功制作C57BL/6小鼠免疫性前列腺炎模型,前列腺炎组织内有明显的炎症细胞浸润;实时定量RT-PCR检测结果显示,免疫性前列腺炎小鼠IL17A、IL23p19、IL23p40等Th17辅助性T淋巴细胞相关细胞因子mRNA相对含量分别为0.053 0±0.026 5、0.017 7±0.006 5、0.028 9±0.028 3;对照组分别为0.003 6±0.0029、0.005 1±0.002 5、0.004 1±0.002 8,免疫性前列腺炎小鼠表达明显增高,与对照组比较差异有统计学意义(P<0.05)。结论Th17辅助性T淋巴细胞相关细胞因子IL17A、IL23等表达明显增高,Th17辅助性T淋巴细胞参与了免疫性前列腺炎的发病。Objective To study the expressions of Thl7 lymphocytes related cytokines including interleukin 17A （IL17A） and interleukin 23 （p19/p40） （IL23p19/p40） in prostate tissue of experimental autoimmune prostatitis（EAP） of C57BL/6 mice. Methods Prostate glands from Wistar rats were used to make purified prostate protein, and then the mice were subcutaneously injected with the purified protein combined with complete Freund＇s adjuvant（CFA） and diphtheria pertussis tetanus （DPT） vaccine at 0 and 30 days. 30 days after the second injection the prostates of the immune mice were observed, and one-step real-time RT-PCR was used to check mRNA expressions of Thl7 lymphocytes related cytokines including IL17A and IL23 （p19/p40） in the prostate tissue of EAP mice, and the differences of the cytokines between the EAP and control groups were compared to study the effectiveness of the cytokines in the onset of autoimmune prostatitis of C57BL/6 mice. Results The mice model of EAP was successfully made. The mRNA relative amounts of IL17A, IL23p19 and IL23p40 in the mice prostate of the EAP and the control group were 0.053 0 ± 0.026 5 vs 0.017 7 ± 0.006 5, 0.028 9 ± 0.028 3 vs 0.003 6 ± 0.002 9, 0.005 1 ± 0.002 5 vs 0.004 1 ± 0.002 8 respectively, and the differences between the two groups were significant （ all P 〈 0.05 ）. Conclusion Expressions of Th17 lymphocytes related cytokines in the prostate tissue of EAP mice are higher than those in the controls, and these cytokines are related to the onset of EAP.

关 键 词：前列腺炎 T淋巴细胞 白细胞介素17 白细胞介素23 

分 类 号：R697.33[医药卫生—泌尿科学]