富勒烯对斑马鱼^60Coγ辐射生物效应修饰作用的研究  

Studies of modifying effects of fullerene on ^60 Co - γ irradiated zebrafish

作　　者：倪瑾[1] 但志刚[1] 高福[1] 孙顶[1] 蔡建明[1] 

机构地区：[1]第二军医大学放射医学教研室,上海200433

出　　处：《中华放射医学与防护杂志》2010年第1期5-8,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金（30200069）;解放军总后军队医药卫生课题资助项目（06MA169）

摘　　要：目的研究富勒烯化合物对斑马鱼受到γ射线照射后的存活率、畸形发生率的影响及其机制，以了解富勒烯类化合物对辐射生物效应的修饰作用。方法^60Coγ射线照射下富勒烯母体纳米颗粒对斑马鱼成鱼存活率的改变，同时利用斑马鱼胚胎-幼体比较该化合物对不同照射条件下斑马鱼鱼卵及幼体畸形发生率的影响，并从活性氧（ROS）生成的改变及DNA链断裂形成探讨了其可能的作用机制。结果光照期给药500×10^-9的富勒烯母体（nano—C60）能增强γ射线照射对成鱼的杀伤作用，在此条件下活性氧生成和DNA损伤增加，且抑制胚胎的孵出率和导致畸形的发生率增加；但避光期用药对射线效应没有明显的增强作用，除了高浓度5000×10^-9会抑制幼鱼的孵出率，并导致发育畸形增加。结论富勒烯母体对斑马鱼的辐射损伤效应有增强作用，其机制可能是在光照期用药后活化并导致活性氧生成增加，并导致DNA氧化损伤增强。Objective To evaluate the modification of C60 on the radiation effects of ^60Co-γ irradiation on zebrafish. Methods The adult and embryonic zebrafish were used as model organisms to examine the potential of C60 to elicit oxidative stress responses on the surviving rate, hatching rate and malformation occurrence, both upon exposure to light or in the dark. Reactive oxygen species （ROS） production and DNA damage were examined as the possible underlying mechanism. Results 500 × 10^-9 nano-C60 waterborne exposure could enhance the γ-irradiation effects by decreasing adult fish survival upon light exposure, which resulted in ROS and DNA damage increasing. The hatching rates were also inhibited with higher malformation, though dark exposure did not make any enhancement, except that the 5000 × 10^-9 C60would inhibit larvae hatching and induced more malformation. Conclusions Waterborne nano-C60 exposure may enhance the radiation effects on zebrafish, ROS production and DNA damage increasing may be the underlying mechanism.

关 键 词：富勒烯 Γ辐射 斑马鱼 辐射修饰 

分 类 号：R144[医药卫生—公共卫生与预防医学]