熊去氧胆酸对肝内胆汁淤积大鼠多药耐药相关蛋白2的影响及意义  

Effect of ursodeoxycholic acid on expression of multidrug resistance-associated protein 2 in alpha-naphthylisothiocyanate-induced cholestatic hepatitis

作　　者：郑曲波[1] 邹越前[1] 程涛[1] 刘惠萍[1] 

机构地区：[1]中国人民解放军第458医院,广东广州510600

出　　处：《中西医结合肝病杂志》2010年第1期41-43,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:制备大鼠肝内胆汁淤积模型,检测熊去氧胆酸干预前后多药耐药相关蛋白2(MRP2)表达的变化。方法:采用Wistar雄性大鼠灌服α-茶基异硫氰酸酯(ANIT)制备肝内胆汁淤积模型,分别以生理盐水、地塞米松、熊去氧胆酸干预大鼠5天后,检测肝功能,并取肝脏,采用实时荧光定量RT-PCR方法检测肝组织MRP2基因mRNA水平,Western-blot检测肝组织内MRP2蛋白水平。结果:与生理盐水组大鼠相比,地塞米松组和熊去氧胆酸组肝功能检测总胆红素、直接胆红素显著降低(P<0.01),肝组织MRP2基因mRNA水平、蛋白水平显著升高(P<0.01)。结论:地塞米松和熊去氧胆酸均能降低胆红素水平,其作用机制可能与两种药物上调MRP2基因表达有关。Objective： To study the effect of ursodeoxycholic acid （UDCA） on expression of multidrug resistance-associated protein 2 （ MRP2 ） in alpha-naphthylisothiocyanare （ANIT） induced cholestatic hepatitis. Methods： Rats were treated 5 days with normal saline, dexamethasone, UDCA respectively after administration of ANIT. The liver function were examined. The expression level of MRP2 on gene and protein were detected by real-time RT-PCR and Western-blot. Results： Compared to the normal saline, dexamethasone and UDCA could significantly decrease the TBil and DBil （ P 〈 0. 01 ）, while markedly increasing the expression of mRNA and protein of MRP2 （P 〈 0. 01 ） . Conclusion： The dexamethasone and UDCA can decrease bilirubin by increasing the expression of MRIY2.

关 键 词：肝内胆汁淤积 熊去氧胆酸 多药耐药相关蛋白2 

分 类 号：R285.5[医药卫生—中药学]