猕猴胚胎干细胞神经分化过程中ASPP家族变化的初步研究(英文)  

Primary research on changes of ASPP family in the process of neural differentiation from Rhesus embryonic stem cells

作　　者：陈锐[1,2] 李荣荣[2] 季维智[2] 张华堂[2] 卢欣[2,3] 

机构地区：[1]中国科学技术大学生命科学学院,安徽合肥230027 [2]中国科学院昆明动物研究所,云南昆明650223 [3]英国牛津大学路德维格肿瘤研究所,英国牛津OX3 7DQ

出　　处：《中国科学技术大学学报》2010年第1期38-47,共10页JUSTC

基　　金：Supported by the CAS/SAFEA International Partnership Programfor Creative Research Team

摘　　要：为研究肿瘤细胞凋亡调控因子ASPP(Apoptosis-stimulating protein of p53)家族蛋白(ASPP1,ASPP2,iASPP)在猕猴神经系统细胞早期发育过程中是否存在变化,并初步研究其变化趋势,通过体外诱导猕猴胚胎干细胞定向分化为神经前体细胞模拟猕猴神经系统细胞早期发育过程,并对此过程中细胞内ASPP蛋白量进行检测,检测方法使用细胞免疫荧光和western blotting.实验初步检测出,肿瘤调控因子ASPP家族蛋白在猕猴神经系统细胞早期发育过程中在蛋白量和蛋白分子量上有变化,并且可以初步了解其变化趋势.该实验结果表明ASPP蛋白家族作为肿瘤细胞凋亡调控因子与猕猴神经系统早期发育过程有着密切的关系,这也许对将来治疗神经系统退行性疾病和肿瘤发生有一定帮助.To research whether there is any change of ASPP（Apoptosis-stimulating protein of p53） family,including ASPP1,ASPP2 and iASPP,during the process of early development of the Rhesus neural system,and to research the tendency of the change primarily,the process of early development of Rhesus neural system was simulated through induced neural differentiation from rESC（Rhesus embryonic stem cells） in vitro;meanwhile,the changes of ASPP family in cells were detected by Immunohistochemical Staining and western blotting.The results show that changes of ASPP family in cells have been detected in the process of induced neural differentiation from rESC（Rhesus embryonic stem cells） in vitro,especially amounts and modifications,and some tendencies of these changes can be indicated from these results.The results suggest that there is some certain connection between ASPP family and early development of Rhesus neural system.It might be helpful for diagnosis and treatment of neuronal degenerative diseases and tumours with neuronal origin in the future.

关 键 词：ASPP1 ASPP2 IASPP 神经前体细胞 猕猴胚胎干细胞 

分 类 号：Q813[生物学—生物工程] Q42