西伯利亚鲟卵巢发育过程血清脂蛋白脂酶活性与血脂代谢的研究  

On lipoprotein lipase activity and lipid metabolism at different ovarian development stages in cultured Acipenser baerii

作　　者：颜世伟[1,2] 庄平[1,2] 张涛[1] 马境[3] 章龙珍[1] 

机构地区：[1]中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室,上海200090 [2]大连水产学院生命科学与技术学院,大连116023 [3]华东师范大学生命科学学院,上海200062

出　　处：《海洋渔业》2010年第1期42-47,共6页Marine Fisheries

基　　金：农业科技成果转化资金项目(2008GB23260408);国家"863"高技术研究发展计划(2008AA10Z227);中央级公益性科研院所基本科研业务费专项资金(2007M01);浙江省科技计划项目(2007C12026);上海教育委员会E-研究院建设资助项目(E03009)

摘　　要：利用微创手术检查的方法对人工养殖西伯利亚鲟的卵巢发育期进行鉴别,根据卵巢发育期将西伯利亚鲟分为Ⅱ期、Ⅲ期、Ⅳ期、Ⅴ期和Ⅵ期共5个实验组。对5个实验组血清脂蛋白脂酶(LPL)活性及血脂含量进行测定,并对其之间的关系进行分析。结果表明:随着卵巢发育,血清LPL活性、极低密度脂蛋白胆固醇(VLDL-C)、甘油三酯(TG)呈"先上升,后下降"的趋势,其中LPL在Ⅴ期达到最高值,VLDL-C、TG均在Ⅳ期达到最高值;总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)随卵巢发育表现为上升趋势。血清TG与LPL呈正相关关系。Ovarian development stages in cultured Acipenser baerii were indentified by minimal invasive surgery. The fish were divided into five groups by ovarian development stages ： stageII , stage III, stage IV, stage V and stage VI. The lipoprotein lipase （LPL） and serum lipid were studied. The results showed： the LPL, very low density lipoprotein cholesterol （VLDL-C）, serum triglyceride （TG） first increased as the development of ovary, thereafter, declined, and the LDL showed highest at stage V and VLDL-C, TG showed highest at stageIV. The TC and LDL-C increased gradually with the development of ovary. The TG showed positive relationship with the activity of LPL.

关 键 词：西伯利亚鲟 卵巢发育 脂蛋白脂酶 血脂代谢 

分 类 号：Q132.7[生物学—普通生物学]