RP-HPLC法测定人血浆中头孢吡肟的含量  

Determination of Cefditoren concentration in Human Plasma by RP-HPLC

作　　者：沈映冰[1] 杨伟权[2] 谢静文[1] 

机构地区：[1]中山大学附属第六医院药剂科,广州510655 [2]中山大学附属第一医院药学部,广州510080

出　　处：《国际医药卫生导报》2010年第4期477-479,共3页International Medicine and Health Guidance News

摘　　要：目的建立RP—HPI．c法测定人血浆巾头孢吡肟的浓度。方法色谱柱：NueleodurC18分析柱（4．6mm×250mm，5μm），流动相：0.03％三氟乙酸缓冲液／乙腈（81／19，V／V，冰醋酸调节pH=5），流速1．0ml／min，检测波长270nm，柱温30℃，进样量20μl。结果头孢吡肟线性范围为0．02～5．0μg／ml。头孢吡肟的最低检测限为0．02μg／ml，日内、日间RSD均小于5％，相对回收率为97．6％～104．5％，提取回收率均大于91．3％。结论该方法灵敏、简便、准确度高，可用于头孢吡肟的血药浓度测定。Objective To establish a RP-HPLC method for determination of Cefepime concentration in human plasma. Methods Nucleodur C18 was used with the mobile phase of 0.03 % trifluoro acetic acid buffer / acetonitrile （81/19, v/v PH=5） at the detection wavelength of 270 nm. The flow rate was 1.0 ml/min. Results Under the condition of this cefditoren in plasma was well separated, the calibration curve was linear in the range of 0.02 - 5.0 μ g/ml. The limit of quantification was 0.02 μg/ml. The RSD of intra- and inter-day were less than 5%, the relative recovery was 97.6% - 104.5% and the extraction recovery was above 91.3%. Conclusion This validated method is sensitive, simple and repeatable enough to he used in pharmacokinetic studies.

关 键 词：头孢吡肟 高效液相色谱 人血浆 

分 类 号：R96[医药卫生—药理学]