几个桃优质新品种的RAPD鉴定与分析  

Identification and analysis on several new peach cultivars by RAPD markers

作　　者：李莉[1] 刘玉祥[2] 彭建营[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]唐山职业技术学院,河北唐山063004

出　　处：《河北农业大学学报》2010年第1期30-33,共4页Journal of Hebei Agricultural University

基　　金：国家自然科学基金资助项目(30270927);河北省自然科学基金项目(C2004000363);国家农业科技成果转化资金项目(2007GB2A200034)

摘　　要：以6个桃品种为研究对象,采用RAPD技术对其进行鉴定和亲缘关系分析。25个随机引物共获得213条DNA谱带,其中65条（30.5%）为多态性带。通过单引物和引物组合可以将6个品种区分开来。RAPD分析结果表明：“秋红晚蜜”和“秋红早蜜”先聚在一起,然后再与“秋红蜜”聚在一起,三者间遗传相似系数为0.983－0.984,为共同起源的地方性品种 “燕红”和“西妃”先聚在一起,然后又与“乐林早红”聚在一起,三者相似系数范围为0.913－0.980,其中“燕红”和“西妃”的相似系数为0.980。推测“西妃”是“燕红”的芽变或者亲本来源相同。In this study, 6 peach cultivars were analyzed using random amplified polymorphic DNA markers. 213 DNA bands were amplified with 25 primers, 65 of which （30.5%） were polymorphic. Six cultivars can be differentiated through single primer and double primers. The result of cluster analysis showed that ＇Qiuhongwanmi＇ and ＇Qiuhongzaomi＇ were clustered first, and then clustered with ＇Qiuhongmi＇, and the genetic similarity coefficients of the three cultivars ranged from 0. 983 to 0. 984; They were local cultivars originated from the same culti- var. ＇ Yanhong＇ and ＇ Xifei ~ were clustered first, and then clustered with ＇ Laolinzaohong＇. The range of genetic similarity coefficients of the three eultivars was from 0. 913 to 0. 980, ＇Yanhong＇ and ＇Xifei＇ of which were 0. 980. ＇Xifei＇ maybe sport from ＇Yanhong＇ or originated from the same parents presumably.

关 键 词：桃 RAPD 品种鉴定 亲缘关系 

分 类 号：S662.1[农业科学—果树学]