菊属植物遗传多样性的RAPD分析  

Genetic diversity of some Dendranthema spp.based on RAPD analysis

作　　者：刘蕤[1] 杨际双[2] 

机构地区：[1]河北农业大学园林与旅游学院,河北保定071001 [2]河北农业大学园艺学院,河北保定071001

出　　处：《河北农业大学学报》2010年第1期60-65,83,共7页Journal of Hebei Agricultural University

基　　金：河北农业大学留学回国人员科研启动基金(2004-897)

摘　　要：采用RAPD分子标记技术分析了菊属10个野生种和12个栽培品种间的遗传关系和多样性。从50个随机引物中筛选出了14条引物,对供试材料的DNA进行扩增,共获得169条清晰可辨的谱带,多态位点比率为96.4%,多态性较高。POPgene32软件计算结果表明：种（品种）间相似系数变幅在0.195 4-0.565 6；平均有效等位基因数为1.515 8,平均Nei’s基因多样性指数为0.321 6,平均Shannon信息指数为0.479 4。并且菊属野生种的多态条带、多态位点比率、平均有效等位基因数（Ne）、平均Nei’s基因多样性指数（He）及Shannon信息指数值均高于栽培菊花,表明野生种的遗传多样性比栽培菊花丰富。根据Jaccard相似系数进行UPGMA聚类结果表明,“若狭滨菊”与栽培菊花关系较近,野菊和小红菊与栽培菊花亦较近.栽培菊花基本可以按照花径聚类。10 wild species and 12 cultivars of Dendranthema were used for analyzing genetic diversity and relationship by RAPD-PCR technology. The fourteen primers screened from 50 random primers, could generate 169 fragments, of which 163 ones （96.4%） were polymorphic, showed high polymorphism in Dendranthema. As analyzed by POPgene32, the similarity coefficient between cultivars ranged from 0. 195 4 to 0. 565 6. The： average value of effective number of alleles, Nei＇s gene diversity and Shannon＇s informa-tion index were 1. 515 8,0. 321 6 and 0. 479 4. The number of polymorphie bands, the average value of effective number of alleles, Nei＇s gene diversity and Shannon＇ s information index in the wild species were higher than the cultivars, showed that genetic diversity in the wild species was larger than the cuhivars. The data were analyzed and clustered using UPGMA method by NTSYS2.0. The results indicated that, the cultivars were most relatively close to D. japonicurn var. wakasaense, secondly, D. indicum and D. chanetii. In the cultivars, the genetic relationship were basically related to their inflorescence size.

关 键 词：菊属 菊花 RAPD 遗传多样性 遗传关系 

分 类 号：S682.1[农业科学—观赏园艺]