致病疫霉拮抗菌梨黑斑病菌发酵条件的优化  

Optimization of fermentation conditions of Alternaria kikuchiana against the growth of Phytophthora infestans

作　　者：刘海珍[1] 蒋继志[1] 王会仙[2] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]河北大学图书馆,河北保定071002

出　　处：《河北农业大学学报》2010年第1期66-69,共4页Journal of Hebei Agricultural University

基　　金：公益性行业(农业)科研专项(3-20);河北省自然科学基金项目(C2007000191)

摘　　要：为提高梨黑斑病菌(Alternaria kikuchiana)102菌株发酵产物对致病疫霉(Phytophthora infestans)Z182菌株生长的抑制效率,本研究利用涂布法和滤纸片法、采用单因素试验设计方法,在静置培养条件下,对梨黑斑病菌的最佳发酵条件进行了优化。结果显示,该菌株在28℃、自然pH、80 mL/250 mL装液量、6%接种量、黑暗培养8 d,所得发酵液对致病疫霉有较强的抑制作用,抑菌率可达85.7%。该菌株的抑制率优化后比优化前高出约15%,表明利用该菌株在抑制致病疫霉生长方面有进一步研究和利用的潜力。In order to enhance the inhibitory effect of substances in fermentation liquid of Alternaria kikuchiana 102 strain against the growth of Phytophthora infestans Z182 strain, fermentation conditions of A. kikuchiana were investigated in this experiment by rubbing method or folter paper method in stationary culture status. The results show that the fermentation liquid at temperature 28℃, the natural pH, broth content 80 mL/250 mL, inoculation amount 6% and darkness for culture 8 d have the strongest inhibitory effect on P. infestans. And the in- hibitory rate reaches 85.7%, an increase by 15% in comparison with the pre-optimized condition, indicating that there is a great potential for using fermentation substances to control potato late blight.

关 键 词：致病疫霉 拮抗作用 梨黑斑病菌 发酵条件优化 

分 类 号：S436.412.1[农业科学—农业昆虫与害虫防治]