不同剂量吡喹酮治疗曼氏裂头蚴感染小鼠的疗效观察  

Therapeutic effect of different dosages of praziquantel on mice infected with Sparganum mansoni

作　　者：李楠[1] 蔺西萌[2] 崔晶[1] 王明明[1] 井丰军[1] 祁欣[1] 王莉 王中全[1] 

机构地区：[1]郑州大学医学院寄生虫学教研室,郑州450052 [2]河南省疾病预防控制中心

出　　处：《中国血吸虫病防治杂志》2010年第1期51-55,共5页Chinese Journal of Schistosomiasis Control

基　　金：河南省医学科技攻关计划项目(200903151)

摘　　要：目的观察不同剂量吡喹酮对曼氏裂头蚴感染小鼠的疗效。方法156只昆明小鼠分2批感染,每只经口(灌胃)接种感染5条裂头蚴。第1批36只小鼠分为6组(每组6只),1-5组小鼠分别接种含不同浓度吡喹酮培养液中培养3d的裂头蚴,6组小鼠接种正常培养液中培养3d的裂头蚴,作为对照组;第2批120只小鼠分成12组(每组10只),分别接种青蛙或蝌蚪体内的裂头蚴,1-9组于感染后1周或5周用不同剂量的吡喹酮灌胃治疗,10-12组为对照组。各组小鼠于治疗结束后1周或2周剖杀,收集裂头蚴数并计算各组的平均检出虫数和减虫率。结果第1批小鼠接种在10-40μg/ml吡喹酮中培养3d的裂头蚴后,检出虫数与对照组相比差异无统计学意义(P>0.05),接种在50μg/ml吡喹酮中培养3d的裂头蚴后,减虫率仅为16.60%,各剂量组减虫率之间差异无统计学意义(P>0.05)。第2批小鼠感染青蛙体内裂头蚴1周后,用200、400、800mg/kg吡喹酮治疗后1周和2周的检出虫数与对照组相比,差异均无统计学意义(P均>0.05),相同剂量组治疗后1周和2周后减虫率差异均无统计学意义(P均>0.05);感染蝌蚪体内裂头蚴1周后,200、400mg/kg吡喹酮治疗后1周检出虫数与对照组相比差异均无统计学意义(P均>0.05),800mg/kg治疗后1周减虫率为17.02%,各剂量组减虫率之间差异无统计学意义(P>0.05)。小鼠感染青蛙体内裂头蚴5周后,用1200、1800mg/kg吡喹酮治疗1周和2周后,检出虫数差异无统计学意义(P>0.05),但均明显高于对照组(P均<0.05),相同剂量组减虫率之间的差异无统计学意义(P>0.05)。结论吡喹酮(10～50μg/ml)在体外对裂头蚴无明显杀伤作用,但大剂量(1800mg/kg)灌胃时对裂头蚴感染小鼠疗效较好。Objective To observe the efficacy of mice infected with Sparganum mansoni by using different dosages of praziquantel. Methods A total of 156 Kunming mice were divided into 2 batches, each of them was orally infected with 5 spargana. Thirty-six mice in the first batch were equally divided into 6 groups, the mice in group 1-5 were inoculated with spargana cultured in different concentrations of praziquantel for 3 days, the group 6 served as a control. One hundred and twenty mice in the second batch were equally divided into 12 groups, each mouse was inoculated with spargana obtained from frogs or tadpoles, group 1-9 were treated by different dosages of praziquantel 1 or 5 weeks post infection, group 10-12 served as controls. All of the mice were sacrificed and dissected 1 or 2 weeks after the treatment, the mean number of worms recovered was calculated and worm reduction rates were determined. Results The number of worm recovered from mice infected with spargana cultured in 10-40 μg/ml of praziquantel had no significant difference with that of the control （ P 〉 0.05 ）. The worm reduction rate was 16.60% while the spargana being cultured in 50 μg/ml of praziquantel. The worm reduction rates of the mice that sacrificed 1 week or 2 weeks after being treated by the same dosage of praziquantel had no significant difference （ P 〉 0.05 ）. When being treated with 200, 400 or 800 mg/kg of praziquantel 1 week post infection, the number of worm recovered from mice infected with spargana from frogs had no significant difference with those of the control 1 and 2 weeks after the treatment （ P 〉 0.05 ）. The worm reduction rates between the groups with the same dosage 1 week and 2 weeks post treatment had no significant difference （ P 〉 0.05 ）. When being treated with 200 or 400 mg/kg of praziquantel 1 week post infection, the number of worm recovered from mice infectedwith spargana from tadpoles had no statistically difference with that of the control 1 week after treatment （ P 〉 O. 05 ）. The worm

关 键 词：曼氏裂头蚴 裂头蚴病 吡喹酮 疗效 

分 类 号：R383.35[医药卫生—医学寄生虫学]