弓形虫感染对大鼠脑组织神经丝mRNA表达的影响  

Effect of Toxoplasma gondii infection on levels of neurofilament mRNA and cellular immunity in rats

作　　者：周永华[1] 胡玉红[2] 顾向明 王玠[1] 黄玉政[1] 董敏[2] 石芳 许永良[1] 高琪[1] 

机构地区：[1]江苏省寄生虫病防治研究所、卫生部寄生虫病预防与控制技术重点实验室,无锡214064 [2]中国人民解放军南京军区总医院 [3]江苏省苏州市吴中区人口与计划生育局

出　　处：《中国血吸虫病防治杂志》2010年第1期63-65,共3页Chinese Journal of Schistosomiasis Control

基　　金：江苏省卫生科研基金(X200739)

摘　　要：目的探讨弓形虫感染对大鼠脑组织神经丝(NF)mRNA表达和细胞免疫水平的影响。方法将4周龄雄性SD大鼠随机分成2组:弓形虫感染组(A组)腹腔感染弓形虫速殖子2×107/ml悬液2ml;正常对照组(B组)腹腔注射灭菌生理盐水2ml。感染弓形虫速殖子9周后,应用逆转录聚合酶链反应检测大鼠大脑组织中高分子量NF(NF-H)、中分子量NF(NF-M)和低分子量NF(NF-L)mRNA表达水平;流式细胞术检测大鼠外周血CD3+、CD4+、CD8+T淋巴细胞;ELISA测定其血清IFN-γ、TNF-α、IL-4等细胞因子。结果大鼠弓形虫感染9周后,大脑组织NF-LmRNA下降为正常对照组的64%(P<0.01);NF-M为96%(P>0.05);NF-H为89%(P<0.05)。感染组大鼠的CD4+和CD8+T淋巴细胞与正常对照组相比,差异均无统计学意义(P均>0.05);感染组大鼠血清中IFN-γ、TNF-α、IL-4等细胞因子的水平均高于正常对照组大鼠水平(P<0.05)。结论弓形虫感染可导致大鼠脑组织中NF亚单位mRNA表达水平的下降,血清IFN-γ、TNF-α、IL-4水平的升高。Objective To investigate the alterations in levels of neurofilament （NF） mRNA and cellular immunity in rats infected with Toxoplasma gondii. Methods Four-week old male SD rats were randomly divided into two groups with ten animals for each. The subjects in the experimental group were injected with 2 × 10^7/ml Toxoplasma tachyzoits 2 ml for each （ Group A ）. Meanwhile, each rat in the control group （ Group B） was injected with NaCl 2 ml. After 9 weeks, mRNA levels of light molecular NF （NF-L）, medium molecular NF （NF-M） and high molecular NF （NF-H） in cerebrum were determined by RT-PCR. The percentages of CD3^＋ , CD4^＋, CD8^＋ T lymphocytes were examined by flow cytometery, and the peripheral blood serum levels of IFN-γ, TNF-α, IL-4 were analyzed by ELISA. Results The mRNA levels of NF-L in rat cerebrum decreased significantly to 64% （P 〈 0.01 ）, NF-M decreased to 96% （ P 〉0. 05 ）, and NF-H declined to 89% （ P 〈 0. 05 ） of the control. No significant changes were found on the levels of CD3 ^＋ , CD4^＋ , CD8^＋ T lymphocytes （ P 〉 0.05 ）. The levels of IFN-γ,TNF-α and IL-4 in the sera of experimental rats were significantly higher than those in the control group （ P 〈 0.05 ）. Conclusion Toxoplasma gondii infection is associated with the alterations in mRNA levels of NF in the cerebrum of rats.

关 键 词：弓形虫 神经丝mRNA 逆转录聚合酶链反应 细胞免疫 大鼠 

分 类 号：R382.5[医药卫生—医学寄生虫学]