萱藻丝状体诱导培养及采苗研究  

Induction and culture of filaments and seedling in sea weed Scytosiphon lomentaria

作　　者：张泽宇[1] 李晓丽[1] 刘宏宇[1] 曹淑青[1] 

机构地区：[1]大连水产学院农业部海洋水产增养殖学重点开放实验室,辽宁大连116023

出　　处：《大连水产学院学报》2010年第1期14-18,共5页Journal of Dalian Fisheries University

基　　金：国家"十一五"支撑计划项目(2006BAD09A01)

摘　　要：对萱藻Scytosiphon lomentaria丝状体诱导培养及采苗进行了研究。在室内培养条件下,从萱藻盘状孢子体上获得了丝状体并游离培养成直径为1～2 cm的藻团,采用将丝状体切碎的方法获得大量盘状孢子体,盘状孢子体经生长后在10℃和15℃下形成单室孢子囊并放出游孢子,以维尼纶绳为附着基进行孢子采集,置于10℃下培养35 d后获得藻体长度为0.5～1.0 cm的萱藻幼苗。结果表明:盘状孢子体在藻体直径达到100μm后开始形成丝状体,20℃下丝状体形成的时间早、数量多,生长速度快,切碎后的丝状体在20℃下5 d后开始形成盘状体,20 d后其形成率达到100%;15℃下10 d后形成盘状体,20 d后其形成率为65%;10℃下12 d后才形成盘状体,20 d后的形成率仅为40%。Induction and culture of filaments and seedling were conducted in sea weed Scytosiphon lomentaria. The filaments were developed into clusters of 1 -2 cm in diameter through the induction and incubation. Massive crusts were yielded from the proliferated filaments and zoospores were released from the mature crusts at 10 ℃ and 15 ℃. The juveniles with the length of 0.5 - 1.0 cm adhered on vinylon strings were obtained in 35 day indoor culture at 10 ℃, indicating that this method provides a new way for nursing the seeding. The filaments were produced from the crusts with diameter over 100 μm, and the optimal formation and best growth of the filaments were observed at 20 ℃. It took only 5 days for the crust formation at 20 ℃, but 10 days at 15 ℃ and 12 days at 10 ℃. The formation rates of the crusts reached up to 100% at 20 ℃, 65% at 15℃ and 40% at 10℃ in 20 day culture.

关 键 词：萱藻 丝状体 室内培养 采苗 

分 类 号：S968.42[农业科学—水产养殖]