金属卟啉与刀豆蛋白A相互作用的室温磷光研究  

Interaction between Metal-porphyrin and Concanavalin A by Room Temperature Phosphorescence

作　　者：王英特[1] 王向伟[1] 高小庆[1] 刘文[1] 武燕妮[1] 

机构地区：[1]山西大学化学化工学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2010年第1期112-115,共4页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(No.20475035);山西省自然科学基金

摘　　要：实验在脱氧胆酸钠盐(NaDC)介导下,采用室温磷光法研究阴离子meso-四-4-(羧基苯基)钯卟啉(Pd-TCPP)与刀豆蛋白A(ConA)之间的相互作用,实验表明Pd-TCPP与ConA之间能形成复合物,结合常数为3.33×104L·mol-1.这一结果可用于利用Pd-TCPP作为室温磷光探针对ConA进行测定.同时,紫外可见光谱和共振光散射光谱也同样证明Pd-TCPP与ConA之间存在相互作用.Room temperature phosphorescence was used to investigate the interaction between Pd-TCPP and ConA. RTP intensity of Pd-TCPP can be quenched by ConA which indicated an obvious interaction between two. The binding constant of this interaction was 3.33×10^4L·mol^-3. UV-Vis spectroscopy and resonance light scattering spectroscopy were also used to testify the conclusion.

关 键 词：meso-四-4-(羧基苯基)钯卟啉 刀豆蛋白A 室温磷光 脱氧胆酸盐 

分 类 号：O657.3[理学—分析化学]