和胃2号对萎缩性胃炎大鼠THBS1甲基化的影响  

Effect of Hewei Decoction 2 on methylation of THBS1 in atrophic gastritis rat

作　　者：韩海明[1] 高志星[2] 季万胜[2] 刘丹丹[2] 王婷[2] 

机构地区：[1]潍坊医学院,山东潍坊261042 [2]潍坊医学院附属医院,山东潍坊261042

出　　处：《现代医药卫生》2010年第5期641-643,共3页Journal of Modern Medicine & Health

摘　　要：目的:观察和胃2号对慢性萎缩性胃炎(CAG)大鼠的治疗作用及THBS1甲基化的影响。方法:复制CAG大鼠模型,和胃2号治疗4周,HE法观察大鼠胃黏膜病理组织学变化,MSP法检测THBS1甲基化。结果:CAG组炎症程度与正常组及和胃2号干预组比较差异均有显著性(P<0.01);THBS1在CAG组甲基化率(15.4%)均高于正常组及和胃2号干预组(P<0.05)。结论:和胃2号对大鼠萎缩性胃炎有改善作用,并可逆转THBS1启动子甲基化。Objective：To study the influence of Hewei Decoction 2 on histopathology of gastric mucosal epithelium and the promoting methylation status of THBS1 in chronic atrophic gastritis （CAG）rat.Methods： We established CAG rats, after Hewei Decoction 2 was used for 4 weeks ,the histopathological changes of gastric mueosal epithelium were observed.The methylation status of THBS1 was examined by methylation-specific polymerase chain reaction （MSP）.Results：Based on inflamnation degree, no significant difference existed between intervention group and normal control group,but there was significant difference between model group and the other two groups.In CAG model group,the frequency of methylation in THBS1 was 15.4%.But there was no THBS1 methylation in intervention group and normal control group.No significant difference existed between intervention group and normal control group.Conclusion：Hewei Decoction 2 can improve the histopathological changes of gastric mucosal epithelium in CAG rat and rerverse THBS1 methylation to stop the canceration of CAG.

关 键 词：和胃2号 萎缩性胃炎 甲基化 THBS1 

分 类 号：R25[医药卫生—中医内科学]