小麦成熟胚脱分化过程中信号转导相关基因的初步分析  

A Preliminary Analysis of Genes Related to Signal Transduction During Dedifferentiation of Mature Embryo in Wheat

作　　者：陈军营[1] 张艳敏[1] 李明杰[1] 马平安[1] 崔琰[1] 陈新建[1] 

机构地区：[1]河南农业大学农学院,郑州450002

出　　处：《中国农业科学》2010年第5期1083-1092,共10页Scientia Agricultura Sinica

基　　金：国家转基因植物研究与产业化专项基金(JY03-B-19-2);河南省杰出人才创新基金资助(022100090)

摘　　要：【目的】研究信号转导相关基因在小麦成熟胚脱分化过程中的表达模式以揭示其脱分化的分子机理。【方法】利用Affymetrix小麦基因芯片研究了小麦成熟胚在MS+2,4-D(2mg·L?1)培养基上脱分化过程不同时间点的基因表达变化,通过NCBI、DATF和DRTF等生物信息学相关网站对基因表达信息进行处理,对信号转导相关基因的变化情况进行了分析,用半定量RT-PCR方法对AF161719.1、BE492852、BJ218430、BJ252470、BJ272518、BJ274015、CA616149、CD896892、CK153462、CK207725、CK171662、CD901339和U48692.1基因进行验证。【结果】有46个信号转导相关基因在小麦成熟胚脱分化过程中的2、6、12、24和72h等不同时间点至少发生了一次有意义的表达变化,其中在整个过程中有32个基因上调,14个基因下调。这些基因包括酪蛋白基因、苏氨酸丝氨酸蛋白激酶基因、14-3-3蛋白基因、钙调素基因和裂原激活蛋白激酶基因,其表达变化与基因芯片的结果基本一致。【结论】CA613597、CA674315、BJ274015、CA654806、CD901339、CK198900、CA697195、CA642143、CA744550和AB011670.1对脱分化的启动和促进可能起重要作用。[Objective] To study the expression profiles of genes related to signal transduction in the mature wheat embryo dedifferentiation to elucidate the molecular mechanism of dedifferentiation. [ Method ] Expression profile of genes in dedifferentiation was studied using Affymetrix microarray technique in mature embryos of wheat cultivar Yumai 18 cultured on MS medium supplemented with 2 mg-L-1 of 2, 4-dichlorophenolxyacetic acid （2, 4-D） at different time points. Their expression information was processed using online tools at NCBI, DATF, and DRTF websites. The expression changes of 13 genes, i.e., AF161719.1, BE492852, BJ218430, BJ252470, BJ272518, BJ274015, CA616149, CD896892, CK153462, CK207725, CK171662. CD901339, and U48692.1, were varified using semi- quantative RT-PCR technique. [Result] Forty-six genes related to signal transduction changed at least at one time point were detected including 32 up-regulated and 14 down-regulated genes during the whole period. They were calcium, serine/threonine protein kinase, casein kinase, protein kinase, 14-3-3 protein, mitogen activated protein kinase. Expression of some genes were confirmed to be consistant with the results of affymetrix microarray. [ Conclusion ] CA613597, CA674315, BJ274015, CA654806, CD901339, CK198900, CA697195, CA642143, CA744550, and AB011670.1 might play an important role in triggering and promoting dedifferentiation of mature embryo in wheat.

关 键 词：信号转导相关基因 小麦成熟胚脱分化 基因芯片 

分 类 号：S512.1[农业科学—作物学]