Ⅹ期鸡胚原壳体外发育影响因素研究  

Influence Factors on Development of Ⅹ-period Chicken Embryos in Primary Eggshell in vitro

作　　者：刘瑞风[1] 刘新峰[1] 秦清明[1] 朱哲[1] 李杰[1] 张荃[1] 闻强[1] 刘丽[1] 郝志明[1] 

机构地区：[1]天津农学院动物科学系,天津300384

出　　处：《中国家禽》2010年第4期22-25,共4页China Poultry

摘　　要：试验以X期鸡胚为材料，研究了翻蛋时间、封口材料及封口胶对X期鸡胚体外发育的影响。试验结果表明：X期鸡胚抽取1mL蛋清后，0d（对照组）、0～3d、3～6d和6-9d不翻蛋组的出雏率分别为33．3％、24．2％、55．9％和23．5％，3-6d不翻蛋组出雏率极显著高于对照组、0～3d及6～9d不翻蛋组（P〈0．01）；3种封口材料中，2种塑料片封口组明显优于蛋壳皿封口组（P〈0．01），塑料输液瓶片封口组的出雏率为16．7％，雪碧饮料瓶片封口组的出雏率为9．5％，而蛋壳皿封口组的出雏率则为0．0％；4种封口胶中自制新胶的效果最好，出雏率高达53．3％；自制水洗胶、自制旧胶和AAA超能胶的出雏率则较低，分别为51．7％、45．0％和40．0％。自制新胶组的出雏率明显优于自制旧胶和AAA超能胶（P〈0．05），而自制新胶与自制水洗胶之间以及自制旧胶与AAA超能胶之间出雏率则没有明显差异（P〉0．05）。The effects of egg-turning time, sealing material and sealing glue on development of X-period chicken embryos in vitro were studied. 1 mL egg white was taken from X-period chicken embryos and which were incubated with no turning. The hatching rates at 0,0 to 3 d,3 to 6 d and 6 to 9 d were 33.3%,24.2%,55.9% and 23.5%, respectively. The hatching rate of no turning egg group at 3 to 6 d was significantly higher than those of no turning egg groups at 0,0 to 3 d and 6 to 9 d （P〈0.01）. Among three sealing materials, the groups with a piece of plastic dropping bottle and sprite bottle were significantly better than the group with a piece of eggshell （P〈0.01）. Hatching rates in the groups with a piece of plastic dropping bottle and sprite bottle were 16.7% and 9.5% respectively,but hatching rate in the group with a piece of eggshell was 0. The hatching rates of the groups with new self-made glue, washed glue, previously seff-made glue and all-purpose adhesive were 53.3%,51.7% ,45.0% and 40.0 %,respectively. There is no difference between new self-made glue group and washed glue group,or between previous self-made glue group and allpurpose adhesive group in hatching rate（P〉0.05）. But hatching rate of new self-made glue group was significantly higher than those of previously self-made glue group and all-purpose adhesive group （P〈0.05）.

关 键 词：X期鸡胚 体外培养 出雏率 翻蛋时间 封口材料 封口胶 

分 类 号：S814.8[农业科学—畜牧学] S858.31[农业科学—畜牧兽医]