白藜芦醇对Hela细胞凋亡及Bcl-2和Fax基因表达的影响  

Efects of resveratrol on apoptosis and expression of Fas and bc1-2 in human cervical cancer Hela cells

作　　者：刘桂艳[1] 唐颖[1] 毛慧玲[1] 张桂荣[1] 刘菲[1] 胡玉红[2] 周学武[1] 崔满华[3] 

机构地区：[1]大庆龙南医院妇产科,大庆163453 [2]佳木斯大学附属第一医院妇产科,佳木斯154001 [3]吉林大学附属第二医院妇产科,吉林130000

出　　处：《中国优生与遗传杂志》2010年第2期17-18,23,共3页Chinese Journal of Birth Health & Heredity

基　　金：黑龙江省自然科学基金资助项目(D2007-82)

摘　　要：目的观察白藜芦醇对人宫颈癌Hela细胞凋亡及Bcl-2、Fax基因表达的影响。方法实验设白藜芦醇用药组和空白对照组,应用流式细胞术检测药物作用24h后细胞凋亡和细胞周期进程情况;应用免疫组化法检测Heal细胞Bcl-2、Fax基因的表达情况。结果经不同浓度的白藜芦醇处理Heal细胞后,镜下可见到凋亡细胞,各组的凋亡率明显高于对照组(P<0.01)。PCM分析发现各实验组S期细胞比例增高,G2/M期细胞比例减少,并呈剂量依赖性(P<0.01);各实验组Heal细胞Bcl-2的表达均低于对照组。而Fax的表达均高于对照组(P<0.01)。结论白藜芦醇对人宫颈癌Hela细胞有明显的抑制生长和促凋亡作用,凋亡途径可能与Fas表达上调、Bel-2表达下调有关。Objective：To investigate effects of resveratrol on the apoptosis and expression of Fas and bcl-2 in human cervical cancer Hela cells. Methods：The experiment included resveratrol treatment groups and control group,the percentage of apoptotic cells and cell cycle progression were measured with FCM at 24 h after resveratrol treatment;The expression of Bcl-2 and Fax of Hela cells were measured with immunohistochemical approach. Results：After Resveratrol treatment,the apoptosis of Hela cells were observed by microscopy,the apoptosis rates of every group were higher than that of control group,the condition of apoptosis were observed by flow cytometry,the number of treated cells in S phase increased,but that in G2/M phase decreased,with a dose dependent fashion （P〈0.01）. The expression of Bcl-2 in treated cells were lower than that of control group,but the Fax were higher than that of control group（P〈0.01）.Conclusions：Resveratrol obviously can inhibit proliferation and induce apoposis of cervical cancer Hela cells. It may be associate with the high expression of Fas and low expression of bcl-2 gene in Hela cells.

关 键 词：白藜芦醇 凋亡 基因 BCL-2 基因  FAX 

分 类 号：R285.5[医药卫生—中药学]