微囊化兔雪旺细胞移植对大鼠损伤脊髓胶质纤维酸性蛋白表达的影响  

Effect of xenotransplantation of microencapsulated Schwann cells on glial fibrillary acidic protein expression in the injured spinal cord of adult rat

作　　者：马建敏[1] 黄建贞[2] 杨宝林[1] 万斌[1] 刘德明[1] 刘曾旭[1] 

机构地区：[1]南昌大学医学院人体解剖学教研室,南昌330006 [2]南昌大学第一附属医院生殖中心,南昌330006

出　　处：《解剖学杂志》2010年第1期73-76,共4页Chinese Journal of Anatomy

基　　金：江西省卫生厅资助项目（20072020）

摘　　要：目的：观察大鼠脊髓损伤后及进行微囊化兔雪旺细胞移植后胶质纤维酸性蛋白（GFAP）表达的变化。方法：130只成年SD大鼠随机分为微囊组、细胞悬液组、损伤对照组和正常对照组4组，术后3、7、14及28d，冰冻切片行免疫组织化学显色观察GFAP表达的变化。结果：大鼠脊髓损伤后3～14d，GFAP阳性细胞数及平均光密度均增加；至第28天时则减少，但仍高于正常组。其中阳性细胞数和平均光密度在第7天开始，微囊组与细胞悬液组、损伤组比较均有明显降低。结论：微囊化异种雪旺细胞移植能抑制损伤脊髓GFAP的表达，减轻由反麻性胶质化所形成的胶质瘢痕。Objective： To study the changes of glial fibrillary acidic protein （GFAP） expression after spinal cord injury （SCI） and the changes after transplantation of microencapsulated Sehwann cells. Methods： 120 adult Sprague-Dawley rats were randomly and equally divided into microcapsule group, cell suspension group, injury group and control group. Additional 10 rats were used as normal controls. The expression of GFAP in sections of rat spinal cord was detected using immnohistochemical technique at 3, 7, 14 and 28 days after injury. Results： After SCI, the number of GFAP positive cells and mean optical density of GFAP were raised from day 3 to day 14, and decreased on day 28, which was still higher than normal. Compared with cell suspension and injury groups, xenotransplantation of microencapsulated Schwann cells produced a signif- icant decrease in the number of GFAP positive cells and mean optical density of GFAP. Conclusion： Xenotransplantation of mieroencapsulated Schwann cells can downregutate the expression of GFAP in astrocytes of the injured spinal cord, there- fore, reduce glial scar caused by reactive astrogliosis.

关 键 词：微囊 脊髓 损伤 雪旺细胞 异种移植 胶质纤维酸性蛋白 

分 类 号：R651.2[医药卫生—外科学] R322.64[医药卫生—临床医学]