两种异位心脏移植小鼠模型的建立和比较  

Establishment and Comparison of Two Mouse Models of Celiac and Cervical Heterotopic Heart Transplantation

作　　者：陈义发[1] 尹辉[2] 梁宾勇[1] 黄致远[1] 刘洪亮[1] 陈孝平[1] 龚非力[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院外科学系,武汉430030 [2]华中科技大学同济医学院免疫学系,武汉430030

出　　处：《中国实验动物学报》2010年第1期17-20,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金资助项目(30571754;30671900);留学回国人员科研启动基金教外司留项目[2005]383号;国家重点基础研究发展规划(973)项目(No.2001cb500008);教育部博士点基金项目(No.20040487056)

摘　　要：目的比较腹部和颈部小鼠心脏移植模型的优缺点,探讨二次器官移植或双心脏移植研究的可行性和实用性动物模型。方法参照Ono法和Chen法并加以改进,建立了同系或者同种小鼠腹部和颈部异位心脏移植模型,同时对两种手术方式的成功率、手术时间、移植心脏存活时间和病理组织学进行了比较。结果腹部和颈部异位心脏移植手术成功率分别为86.7%和83.3%,两种手术方式在总手术时间、移植心脏存活时间和病理组织形态学检查上均无明显差异(P>0.05)。结论在熟练掌握显微外科技术的基础上,两种异位心脏移植模型都能顺利建立,两者既可分开选用,又可结合运用,以适应不同实验需求。Objective Objective In order to better keep up with the development of transplantation immunobiology,we established and compared two types of mouse heterotopic heart transplantation,and hope to help further organ transplantation studies.Methods According to the surgical procedures of Ono＇s type and Chen＇s type of mouse model of heterotopic heart transplantation with some modification,we performed celiac and cervical heteropotic heart transplantation between iso-strains and hetero-strains,and compared the operation success rate,operation time,allograft survival time,and histopathology of those establishment methods.Results The success rates of mouse celiac and cervical heterotopic heart transplantation were 86.7% and 83.3%,respectively,with a non-significant difference（P〉0.05）between the two methods of operation regarding the total operation time,survival time of the allografts,and histopathological findings.Conclusions Based on the mastery of microsurgical techniques,the two models of heterotopic mouse heart transplantation can be established equally,and either of them can be considered depending on the particular requirements of studies.

关 键 词：心脏移植 动物模型 小鼠 

分 类 号：R654.28[医药卫生—外科学]