西藏小型猪细胞色素b基因序列的比较  

Comparison of the Cyt b Gene Sequences in Tibet Mini-Pig and Other Chinese Pigs

作　　者：李洪涛[1] 肖东[2] 顾为望[2] 吴清洪[2] 袁进[2] 王万山[2] 张嘉宁[2] 

机构地区：[1]广州医学院第一附属医院,广州510120 [2]南方医科大学实验动物中心,广州510515

出　　处：《中国实验动物学报》2010年第1期44-47,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：广东省科研计划项目(编号:2006B60101059;2007B020706002)

摘　　要：目的对西藏小型猪Cyt b-基因序列进行分析,研究其遗传背景及其与国内家猪的亲缘关系。方法提取西藏小型猪和巴马小型猪、贵州香猪、五指山猪的全基因组DNA,设计引物扩增Cyt b基因,测序后进行碱基序列比对,建立亲缘关系树,分析西藏小型猪的遗传背景。结果西藏小型猪等国内部分品种猪的Cyt b基因序列与欧洲猪相比有14个变异位点;但是西藏小型猪与国内品种猪相比存在两个特殊碱基位点即在420位点T→C转换的同时在883位点存在G→A转换。结论西藏小型猪与巴马小型猪、贵州香猪、五指山猪等国内家猪有很近的亲缘关系。同时进一步证实西藏小型猪群体内存在一定的遗传分化。Objective To analyze the Cyt b gene sequences in Tibet mini-pigs and clarify the differences and genetic relationship with other Chinese pigs. Method The sequence of Cyt b gene was amplified from genome DNA of Tibet mini-pig,Bama miniature pigs,Guizhou xiang pigs and Wuzhishan（WZS） pigs.After sequencing,the base sequences were compared and analysed.The blood relationship tree and evolution position of Tibet mini-pig were established. Result There were 14 mutation sites between domestic pigs in China and pigs from Europe.Besides there was a significant difference in two nucleotide site ： a T→C switch in site 420 and the G→A switch in site 883 at the same time.Conclusion Chinese pigs include Bama miniature pigs, Guizhou xiang pigs and WZS pigs,have a very close blood relationship with some of Tibet mini-pigs.It has been confirmed that there is a certain genetic differentiation in the Tibet mini-pig.

关 键 词：西藏小型猪 细胞色素B基因 比较研究 

分 类 号：R332[医药卫生—人体生理学]