影响小鼠孤雌胚胎干细胞的建系效率因素初探  

Analysis of Factors Affecting the Efficiency of Establishment of Mouse Parthenogenetic Embryonic Stem Cells

作　　者：陈志胜[1] 吴浩佳[2] 刘林[3] 

机构地区：[1]佛山科学技术学院动物医学系,佛山528000 [2]中山大学生命科学学院,广州510275 [3]南开大学生命科学学院,天津300071

出　　处：《中国实验动物学报》2010年第1期52-55,I0006,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：广州市科技发展计划重点项目(编号:2005Z2-E0141)

摘　　要：目的检测孤雌胚胎干细胞系的建系效率与小鼠品系以及培养体系的关系。方法将小鼠MⅡ期卵子孤雌激活发育至囊胚,然后从囊胚内细胞团分离孤雌胚胎干细胞。结果杂交和近交系小鼠的建系效率没有显著差异,建系的培养体系中加入ERK抑制剂或者采用血清替代品KSR时,建系效率显著提高。结论小鼠孤雌胚胎干细胞的建系效率与小鼠的遗传背景并没有直接关系,而与分离内细胞团的培养体系密切相关。Objective To study the factor affecting the efficiency of establishment of parthenogenetic stem cells（PESCs） in mice.Methods PESC lines were derived from parthenogenetically activated blastocyst inner cell mass of different mice strains and cultured in different systems.Results The efficiency of establishing the pESC showed no significant difference in hybrid and inbred lines.But the efficiency was increased in culture systems added with ERK inhibitor or knockout serum replacement（KSR）.Conclusion The efficiency of establishment of pESC was not directly related with the genetic background of mice but it was closely related to the culture systems.

关 键 词：小鼠 孤雌发育 胚胎干细胞 

分 类 号：Q-28[生物学]