基质金属蛋白酶-3在慢性牙周炎孕鼠模型中的表达  

Expression of matrix metalloproteinase-3 in placenta tissue of pregnant rats chronic peridontitis model

作　　者：史芳川[1] 钟良军[1] 王进涛[1] 张源明[2] 徐隽[1] 倪佳[1] 

机构地区：[1]新疆医科大学第一附属医院口腔科,乌鲁木齐830054 [2]新疆医科大学第一附属医院心脏中心,乌鲁木齐830054

出　　处：《口腔医学》2010年第2期70-72,92,共4页Stomatology

基　　金：国家自然科学基金项目(30860314);新疆维吾尔自治区自然科学基金项目(200821112);新疆维吾尔自治区科技攻关和重点项目(200733146)

摘　　要：目的构建孕鼠慢性牙周炎模型,探讨孕鼠慢性牙周炎胎盘组织、血清及羊水中基质金属蛋白酶-3的表达。方法20只Wistar雌鼠随机分为两组:正常组(n=8)、实验组(n=12),各组接受相应的建模处理。观察其牙周组织的病理改变,实时荧光定量PCR分析胎盘组织中基质金属蛋白酶-3的表达,酶联免疫吸附实验检测血清及羊水基质金属蛋白酶-3含量。结果实验组牙周组织改变:牙槽骨吸收,牙周袋形成,组织病理见有大量的破骨细胞、附着丧失。实时荧光定量PCR及酶联免疫吸附试验检测基质金属蛋白酶-3,两组有差距有统计学意义。结论慢性牙周炎可以促进基质金属蛋白酶-3的合成及分泌,并进入血循环通过胎盘屏障,增加不良妊娠的机率。Objective To detect and analyze the espression of matrix metalloproteinase-3 in blood - serum and amniotic fluid of construction of pregnant rats chronic peridontitis model. Methods 20 wistar female rats were randomly divided into two groups： the normal group （n = 8 ） and the experimental group （n = 12）. Every group accepted the corresponding measurements to establish an animal model. To observe the periodontal tissues and pathology changes, the expression of matrix metalloproteinase-3 in placenta tissue was tested by Real-time PCR. ELISA was employed to detect the content of matrix metalloproteinase-3 in blood-serum and amniotic fluid. Results In frontal-resorption and periodontal pocket, a large number of osteoelasts and attachment loss were found in histopatbology. The content of matrix metallo-proteinase-3 was obviously different in two sets （ P 〈 0.05 ）. Concluctions The chronic periodontitis may high-regulate the expression of matrix metalloproteinase-3 into blood circulation through placental barrier.

关 键 词：慢性牙周炎 孕鼠动物模型 基质金属蛋白酶-3 

分 类 号：R349.63[医药卫生—基础医学]