沙棘痕量挥发物采集方法探讨及成分分析  

Study on Collection Method and Component Analysis of Trace Volatiles from Hippophae rhamnoides

作　　者：刘书景[1] 金幼菊[1] 宗世祥[2] 骆有庆[2] 姚国龙 李元 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]北京林业大学森林培育与保护教育部重点实验室,北京100083 [3]宁夏彭阳县林业局,宁夏756500

出　　处：《中国农学通报》2010年第5期77-81,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"沙棘木蠹蛾灾害发生机制研究"(30730075);长江学者与创新团队发展计划(PCSIRT0607)

摘　　要：沙棘挥发物释放量极少,在采集和测定过程中,本底干扰是影响其精确定量的关键因子。针对采样装置中连接管路气味物质对沙棘挥发物的严重干扰,此研究对五种不同产地、不同材质的连接管进行挥发物采集,并通过热脱附-气相色谱-质谱联用(TCT-GC-MS)进行测定。结果表明,美国产食品级聚氯乙烯管(PVC)杂质峰最小,比较适宜采集沙棘痕量挥发物。该方法可以降低干扰、提高信噪比,为低释放型植物挥发物的采集分析提供了新的参考依据。在近自然状态下,壬烷、辛烷、3-甲基丁醇、戊醛、2-甲基丁醛、2-壬烯醇、2-苯甲酸叶醇酯为沙棘枝叶主要挥发物组分。Leaves of tlippophae rhamnoides normally release small quantities of volatile chemicals. In volatiles collection and determination, the interference of background is the key factor influencing accurate quantitative analysis. For the great interference of odor from connecting pipe to volatiles, volatiles from Hippopbae rhamnoldes were collected using five connecting pipe from different places and material and their components were analyzed using TCT-GC/MS. The results indicated that, the impurity peaks from PVC made in USA were minimized. It is suitable for collection of trace volatiles. The method can reduce interference and raise signal-to-noise ratio. The method will supply a new reference for collecting and analyzing volatiles of low-release plants.Furthermore,the main volatiles from e rharanoides included Nonane, Octane, 3-methyl-l-Butanol, Pentanal, 2-methyl- Butanal, 2-Hexen-l-ol, 2-Hexenyl benzoate in close natural state.

关 键 词：沙棘 痕量挥发物 食品级PVC管 TCT-GC.MS 

分 类 号：S3[农业科学—农艺学]