长白猪和梅山猪脂肪中脂肪沉积相关基因的表达差异  

Expression Changes of Genes Related to Adipose Deposition during Postnatal Development of Backfat in Landrace and Meishan Pigs

作　　者：张凯[1] 顾以韧[1] 刘艾晶[2] 蒋岸岸[1] 帅素容[1] 李明洲[1] 李学伟[1] 

机构地区：[1]四川农业大学动物科技学院,雅安625014 [2]四川农业大学动物医学院,雅安625014

出　　处：《畜牧兽医学报》2010年第2期129-134,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：转基因生物新品种培育重大专项(2009ZX08009-155B;2008ZX08006-003);国家公益性行业(农业)科研专项(NYHYZX07-034)资助

摘　　要：为了揭示瘦肉型的长白猪和脂肪型的梅山猪脂肪沉积相关基因的表达差异,采用qRT-PCR方法检测了两猪种背部皮下脂肪组织中硬脂酰辅酶A去饱和酶（SCD）、苹果酸酶（ME1）和解耦联蛋白3（UCP3）基因的表达量在30、60、90、120和150 d的变化。结果表明,两品种SCD的表达模式除90~120 d相反外,其他各日龄之间基本相同;ME1在30~120 d基本相同,而120~150 d呈现出相反的模式;UCP3除120~150 d相同外,其他各日龄之间呈现相反的模式。以上结果初步揭示了两猪种在生长发育过程中SCD、ME1和UCP3表达的发育性变化模式和品种差异,为深入研究脂肪合成代谢的调控机制提供了基础数据。To investigate the differential expression patterns of genes related to adipose deposition between Landrace（a leaner,Western breed） and Meishan pigs（a fatty,indigenous,Chinese breed）.The expression changes of stearoyl-CoA desaturease（SCD）,cytoplasmic malic enzyme（ME1）and uncoupling protein 3（UCP3）genes were measured in two pig breeds at five growth stages（30,60,90,120 and 150 days） by using qRT-PCR approach.The results showed that the similar expression pattern of SCD mRNA was observed except from 90 to 120 days between the two pig breeds.Expression change of ME1 mRNA was similar between two pig breeds from 30 to 120 days,but the opposite expression pattern was exhibited during 120 to 150 days.Expression changes of UCP3 mRNA in two pig breeds were opposite except from 120 to 150 days.These results suggested that the mRNA level of SCD,ME1 and UCP3 genes in backfat tissue are different in postnatal developmental stages between Landrace and Meishan pigs,which provided some data for further study on the three genes for elucidating the molecular mechanism of fat deposition.

关 键 词：猪 背部皮下脂肪 荧光实时定量PCR 硬脂酰辅酶A去饱和酶(SCD)基因 苹果酸酶(ME1)基因 解耦联蛋白3(UCP3)基因 

分 类 号：S828[农业科学—畜牧学] S813.1[农业科学—畜牧兽医]