表现腺胃炎的蛋用型鸡J亚群-白血病病毒的分离与鉴定  

Subgroup J Avian Leukosis Viruses Isolated from Egg-type Chickens with Proventriculitis

作　　者：孙淑红[1] 柴家前[1] 王波[1] 孙洪磊[1] 王晓云[1] 崔治中[1] 

机构地区：[1]山东农业大学动物科技学院/动物医学院,泰安271018

出　　处：《畜牧兽医学报》2010年第2期251-254,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：山东省中青年科学家基金(2008BS07004);山东省农业重大应用技术创新课题(2007);山东农业大学青年科技创新基金

摘　　要：从表现腺胃炎的尼克珊瑚粉商品代蛋鸡中分离到J亚群-白血病病毒(ALV-J)。将病料或鸡白细胞接种于CEF,培养12 d,分别采用单克隆抗体间接免疫荧光试验检测,结果10只鸡中有9只鸡分离到ALV-J,其中有4只鸡还存在与禽网状内皮增生病病毒(REV)的共感染。通过PCR扩增gp85基因,与已发表的20株ALV-J进行同源性比较。结果表明,与来自白羽肉鸡的HPRS103的同源性为97.8%,而与来自蛋用型鸡的SD07LK1株的同源性为93.0%。本研究发现,在某些仅仅发生腺胃炎的鸡也可能普遍存在ALV-J感染,再次显示了腺胃炎病料中病毒感染的多样性。ALV-J可能成为致腺胃炎的病原之一,但其致病作用有待进一步研究。Subgroup J avian leukosis virus（ALV-J） was isolated from both provetriculus suspensions and blood samples of 9 in 10 laying hens only with proventriculitis,and co-infection of ALV-J with reticuloendotheliosis virus was also confirmed in 4 chickens.The gp85 genes of ALV-J isolates were amplified by PCR,sequenced and compared to 20 reference ALV-J strains.Sequence comparisons indicated that the provetriculus isolate had 97.8% identity with the HPPS103 strain isolated from white meat-type chickens and 93.0% identity with SD07LK1 strain from egg-type chickens.The result demonstrated that ALV-J infection was common in some chickens with only proventriculus,further indicating the diversity of virus infections associated with proventriculitis.ALV-J may be take part in pathogenesis of provetriculitis in some chickens,but its pathogenic role needs to be further studied.

关 键 词：腺胃炎 蛋用型鸡 J亚群-禽白血病病毒 

分 类 号：S852.659.3[农业科学—基础兽医学]