苯丁酸钠对人舌鳞癌Tca8113细胞株p21和survivin基因表达的影响  

The effects of sodium phenylbutyrate(SPB)on the expression of p21 and survivine genes on human tongue squamous cancer cell line Tca8113

作　　者：潘德顺[1] 陈伟强[2] 

机构地区：[1]广东药学院药理学教研室,广东广州510006 [2]广东药学院病理生理学教研室,广东广州510006

出　　处：《中国药理学通报》2010年第2期178-181,共4页Chinese Pharmacological Bulletin

基　　金：中国-意大利科技合作项目(No200625)

摘　　要：目的研究苯丁酸钠对人舌鳞癌Tca8113细胞株的生长、凋亡及其对p21和survivin分子表达的影响。方法应用MTT法检测苯丁酸钠对人舌鳞癌Tca8113细胞株的抑制作用,采用流式细胞仪研究苯丁酸钠对人舌鳞癌Tca8113细胞株的细胞周期阻滞和诱导凋亡作用,应用Western blot和RT-PCR检测p21和survivin基因的转录和表达。结果实验表明苯丁酸钠对Tca8113细胞株增殖有明显的抑制作用;流式细胞仪检测显示苯丁酸钠诱导Tca8113细胞株G1期阻滞和细胞凋亡;苯丁酸钠能使p21的mRNA及蛋白质表达升高,同时survivin的mRNA及蛋白质表达下降。结论苯丁酸钠通过刺激p21表达增加和抑制survivin的表达,从而抑制Tca8113细胞株增殖,诱导细胞G1期阻滞和细胞凋亡;并且p21mRNA水平的上升和survivin mRNA水平的下降变化呈负相关(rs=-0.548,P<0.01),二者蛋白表达的变化亦如此(rs=-0.514,P<0.01)。Aim To investigate the effects of sodium phenylbutyrate on cell proliferation, apoptosis and its expression of p21 and survivin genes in human tongue squamous cancer Tca8113 cell line. Methods The cellular proliferation inhibitory ratio was evaluated by MTT assay and the apoptosis and cell cycle of the Tca8113 cell line was detected by FCM. The expression of p21 and survivin genes was analyzed with Western blot and RT-PCR. Results Sodium phenylbutyrate could inhibit the Tca8113 cells proliferation, promote cell apoptosis and arrest the cells at G1/G0 phase. The expression of p21 gene in TcaS113 cell line treated bysodium phenylbutyrate was increased, and one of survivin gene was decreased. Conclusions Sodium phenylbutyrate induces up-regulation of p21 gene and down-regulation of survivin gene, which inhibits Tca8113 cell proliferation and induces its apoptosis and arrests the cells at G1/Go phase. And the increase of p21 mRNA expression is negatively correlated with the decrease of survivin mRNA expression （ rs = - 0. 548, P 〈0. 01 ） ,and so is its protein expression（rs = -0. 514, P〈0. 01）.

关 键 词：苯丁酸钠 P21 SURVIVIN TCA8113细胞株 凋亡 基因表达 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学] R329.28[医药卫生—基础医学]